基于相关序列扩增多态性分子标记的桂花栽培品种演化分析
为了进一步揭示桂花栽培品种的演化历程，为桂花育种工作提供借鉴，采用相关序列扩增多态性分子标记技术和毛细管电泳技术，对45份桂花材料进行遗传多样性和群体结构分析，以木犀属中最为原始的牛矢果（Osmanthusmatsumuranus）为外部群体，构建桂花栽培品种系统发生树，计算不同演化水平两性花品种的比例，分析桂花性别系统进化情况。结果显示：10对高多态性引物共检测到137个多态性位点，平均每对引物13.7个位点，多态性信息含量为0.2028～0.3027；Nei遗传多样性指数为0.2203～0.3502；Shannon多样性信息指数为0.3483～0.5193。45份桂花材料分为7个亚群和1个...
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| Published in | 浙江大学学报（农业与生命科学版） Vol. 43; no. 4; pp. 404 - 415 |
|---|---|
| Main Author | |
| Format | Journal Article |
| Language | Chinese |
| Published |
杭州市园林绿化股份有限公司,杭州,310020%浙江理工大学建筑工程学院,杭州,310018
2017
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| Subjects | |
| Online Access | Get full text |
| ISSN | 1008-9209 |
| DOI | 10.3785/j.issn.1008-9209.2016.08.241 |
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| Abstract | 为了进一步揭示桂花栽培品种的演化历程，为桂花育种工作提供借鉴，采用相关序列扩增多态性分子标记技术和毛细管电泳技术，对45份桂花材料进行遗传多样性和群体结构分析，以木犀属中最为原始的牛矢果（Osmanthusmatsumuranus）为外部群体，构建桂花栽培品种系统发生树，计算不同演化水平两性花品种的比例，分析桂花性别系统进化情况。结果显示：10对高多态性引物共检测到137个多态性位点，平均每对引物13.7个位点，多态性信息含量为0.2028～0.3027；Nei遗传多样性指数为0.2203～0.3502；Shannon多样性信息指数为0.3483～0.5193。45份桂花材料分为7个亚群和1个混合群体，亚群间遗传分化明显，基因交流较少。系统发生树表明，桂花栽培品种演化历程大致分为10个阶段，大部分银桂品种和四季桂品种最先形成，雄性丹桂品种最晚形成，其他品种处于演化的中间阶段。两性花品种比例随着演化水平的增高呈现逐渐降低的趋势，证明桂花的雄全异株性别系统是雌雄同花演化为雌雄异株的中间状态。此外，桂花花色连续变异可能受到多个CCDs家族同源基因突变的影响，而遗传漂变导致新等位基因丢失，这可能是野生桂花罕见橙红色个体的原因。 |
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| AbstractList | Q37; 为了进一步揭示桂花栽培品种的演化历程,为桂花育种工作提供借鉴,采用相关序列扩增多态性分子标记技术和毛细管电泳技术,对45份桂花材料进行遗传多样性和群体结构分析,以木犀属中最为原始的牛矢果(Osmanthus matsumuranus)为外部群体,构建桂花栽培品种系统发生树,计算不同演化水平两性花品种的比例,分析桂花性别系统进化情况.结果显示:10对高多态性引物共检测到137个多态性位点,平均每对引物13.7个位点,多态性信息含量为0.2028～0.3027;Nei遗传多样性指数为0.2203～0.3502;Shannon多样性信息指数为0.3483～0.5193.45份桂花材料分为7个亚群和1个混合群体,亚群间遗传分化明显,基因交流较少.系统发生树表明,桂花栽培品种演化历程大致分为10个阶段,大部分银桂品种和四季桂品种最先形成,雄性丹桂品种最晚形成,其他品种处于演化的中间阶段.两性花品种比例随着演化水平的增高呈现逐渐降低的趋势,证明桂花的雄全异株性别系统是雌雄同花演化为雌雄异株的中间状态.此外,桂花花色连续变异可能受到多个CCDs家族同源基因突变的影响,而遗传漂变导致新等位基因丢失,这可能是野生桂花罕见橙红色个体的原因. 为了进一步揭示桂花栽培品种的演化历程，为桂花育种工作提供借鉴，采用相关序列扩增多态性分子标记技术和毛细管电泳技术，对45份桂花材料进行遗传多样性和群体结构分析，以木犀属中最为原始的牛矢果（Osmanthusmatsumuranus）为外部群体，构建桂花栽培品种系统发生树，计算不同演化水平两性花品种的比例，分析桂花性别系统进化情况。结果显示：10对高多态性引物共检测到137个多态性位点，平均每对引物13.7个位点，多态性信息含量为0.2028～0.3027；Nei遗传多样性指数为0.2203～0.3502；Shannon多样性信息指数为0.3483～0.5193。45份桂花材料分为7个亚群和1个混合群体，亚群间遗传分化明显，基因交流较少。系统发生树表明，桂花栽培品种演化历程大致分为10个阶段，大部分银桂品种和四季桂品种最先形成，雄性丹桂品种最晚形成，其他品种处于演化的中间阶段。两性花品种比例随着演化水平的增高呈现逐渐降低的趋势，证明桂花的雄全异株性别系统是雌雄同花演化为雌雄异株的中间状态。此外，桂花花色连续变异可能受到多个CCDs家族同源基因突变的影响，而遗传漂变导致新等位基因丢失，这可能是野生桂花罕见橙红色个体的原因。 |
| Abstract_FL | Sweet osmanthus (Osmanthus fragrans) is one of the top ten famous native horticultural plants in China. According to different flowering seasons, flower colors and inflorescence types, the cultivars are divided into four groups:O. fragrans Asiaticus Group, O. fragrans Albus Group, O. fragrans Luteus Group and O. fragrans Aurantiacus Group. Despite long-term cultivation of Osmanthus, little information was recorded on the formation of so many cultivars. O. fragrans Asiaticus Group was considered as the most primitive cultivars, and the ones with light color flowers formed earlier, then followed by deep color flowers. The cluster results based on various types of molecular markers were quite different from traditional classification system, indicating diversity of phenotypic traits might account for a tiny part of the whole genetic diversity of sweet osmanthus. However, at present, few studies can give evidence to further understand the evolution process of sweet osmanthus cultivars. In this study, to further understand the evolution theory, a rooted phylogenetic tree for the cultivars of sweet osmanthus was constructed based on population structure analysis through sequence-related amplified polymorphism (SRAP) technology. Forty-five cultivars were used as plant materials;O. heterophyllus, O. fordii, O. cooperi"Yujie"and O. cooperi"Xuegui"were used as controls, and O. matsumuranus was used as an outgroup. Ten pairs of SRAP primers with high polymorphism were applied to amplify DNA of all samples, and the fragments were examined by capillary electrophoresis. POPGENE 1.32 software was applied to analyze genetic diversity and genetic differentiation. Structure 2.34 software was used to analyze population structure and divide cultivars into subgroups. Nei's genetic distance among subgroups was calculated by NTSYSpc, then applied to construct a rooted phylogenetic tree by MEGA 6. The rate of hermaphrodite flower cultivars on each level of the phylogenetic tree was calculated to understand the sexual system evolution. Moreover, the genetic mechanism of flower color variation for sweet osmanthus was further speculated based on the result. Results showed that the 10 pairs of SRAP primers produced 137 polymorphic bands among all the samples with an average of 13.7 bands per primer. Polymorphism information content ranged from 0.2028 to 0.3027, with an average of 0.2507. Nei's genetic diversity index ranged from 0.2203 to 0.3502, with an average of 0.2835. The Shannon's genetic diversity index ranged from 0.3483 to 0.5193, with an average of 0.4364. There was significant population structure among sweet osmanthus cultivars, and 36 cultivars could be divided into seven subgroups with simple genetic background. Nine cultivars had complicated genetic background, which were identified as a mixed group. Gene differentiation coefficient (Gst) was 51.32％among subgroups, much higher than that of four cultivar groups. Moreover, less gene flow was observed among subgroups than that of four cultivar groups. These results indicated that the cultivars in the same subgroup had much closer genetic relationship than those in the same cultivar group. Using subgroups as the unit of evolution, a rooted phylogenetic tree was constructed. The sweet osmanthus cultivation had experienced about 10 stages (A-J level):subgroup 3 composed of major cultivars in O. fragrans Asiaticus Group and O. fragrans Albus Group formed first, and subgroup 5 composed of the male cultivars in O. fragrans Aurantiacus Group formed the latest, and the cultivars in O. fragrans Luteus Group formed in each stage after D level. With the evolution process, the rate of hermaphrodite flower cultivars dramatically reduced, proving that androdioecy sexual system of sweet osmanthus originated from monoecism. Moreover, the flower color of sweet osmanthus may be controlled by multiple homologous genes in the CCDs family, and the mutations resulted in flower color changing continuously from white to orange red. Loss of new alleles due to genetic drift led to rare individuals with orange red flower in wild population. In conclusion, a new efficient method is offered to further understand the process for formation of sweet osmanthus cultivars and provide a significant reference for genetic mechanism study on evolution of flower colors. |
| Author | 邱帅;吴光洪;陈徐平;郭娟;魏建芬;沈柏春;胡绍庆 |
| AuthorAffiliation | 杭州市园林绿化股份有限公司,杭州310020;浙江理工大学建筑工程学院,杭州310018 |
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| Author_FL | WU Guanghong HU Shaoqing CHEN Xuping QIU Shuai SHEN Baichun GUO Juan WEI Jianfen |
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| DocumentTitleAlternate | Evolution analysis of sweet osmanthus(Osmanthus fragrans)cultivars based on sequence-related amplified polymorphism molecular marker |
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| Keywords | 相关序列扩增多态性 桂花 sweet osmanthus (Osmanthus fragrans) population structure evolution sequence-related amplified polymorphism 群体结构 演化 |
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| Notes | Sweet osmanthus(Osmanthus fragrans)is one of the top ten famous native horticultural plants in China.According to different flowering seasons,flower colors and inflorescence types,the cultivars are divided into four groups:O.fragrans Asiaticus Group,O.fragrans Albus Group,O.fragrans Luteus Group and O.fragrans Aurantiacus Group.Despite long-term cultivation of Osmanthus,little information was recorded on the formation of so many cultivars.O.fragrans Asiaticus Group was considered as the most primitive cultivars,and the ones with light color flowers formed earlier,then followed by deep color flowers.The cluster results based on various types of molecular markers were quite different from traditional classification system,indicating diversity of phenotypic traits might account for a tiny part of the whole genetic diversity of sweet osmanthus.However,at present,few studies can give evidence to further understand the evolution process of sweet osmanthus cultivars.In this study,to further understand the evolution |
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| Publisher | 杭州市园林绿化股份有限公司,杭州,310020%浙江理工大学建筑工程学院,杭州,310018 |
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| Snippet | ... Q37; 为了进一步揭示桂花栽培品种的演化历程,为桂花育种工作提供借鉴,采用相关序列扩增多态性分子标记技术和毛细管电泳技术,对45份桂花材料进行遗传多样性和群体结构分析,以木犀属中最为原始的牛矢果(Osmanthus... |
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| SubjectTerms | 桂花;相关序列扩增多态性;群体结构;演化 |
| Title | 基于相关序列扩增多态性分子标记的桂花栽培品种演化分析 |
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