基于相关序列扩增多态性分子标记的桂花栽培品种演化分析

为了进一步揭示桂花栽培品种的演化历程，为桂花育种工作提供借鉴，采用相关序列扩增多态性分子标记技术和毛细管电泳技术，对45份桂花材料进行遗传多样性和群体结构分析，以木犀属中最为原始的牛矢果（Osmanthusmatsumuranus）为外部群体，构建桂花栽培品种系统发生树，计算不同演化水平两性花品种的比例，分析桂花性别系统进化情况。结果显示：10对高多态性引物共检测到137个多态性位点，平均每对引物13.7个位点，多态性信息含量为0.2028～0.3027；Nei遗传多样性指数为0.2203～0.3502；Shannon多样性信息指数为0.3483～0.5193。45份桂花材料分为7个亚群和1个...

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Published in浙江大学学报（农业与生命科学版） Vol. 43; no. 4; pp. 404 - 415
Main Author 邱帅;吴光洪;陈徐平;郭娟;魏建芬;沈柏春;胡绍庆
Format Journal Article
LanguageChinese
Published 杭州市园林绿化股份有限公司,杭州,310020%浙江理工大学建筑工程学院,杭州,310018 2017
Subjects
桂花;相关序列扩增多态性;群体结构;演化
相关序列扩增多态性
桂花
sweet osmanthus (Osmanthus fragrans)
population structure
evolution
sequence-related amplified polymorphism
群体结构
演化
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ISSN1008-9209
DOI10.3785/j.issn.1008-9209.2016.08.241

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Summary:为了进一步揭示桂花栽培品种的演化历程，为桂花育种工作提供借鉴，采用相关序列扩增多态性分子标记技术和毛细管电泳技术，对45份桂花材料进行遗传多样性和群体结构分析，以木犀属中最为原始的牛矢果（Osmanthusmatsumuranus）为外部群体，构建桂花栽培品种系统发生树，计算不同演化水平两性花品种的比例，分析桂花性别系统进化情况。结果显示：10对高多态性引物共检测到137个多态性位点，平均每对引物13.7个位点，多态性信息含量为0.2028～0.3027；Nei遗传多样性指数为0.2203～0.3502；Shannon多样性信息指数为0.3483～0.5193。45份桂花材料分为7个亚群和1个混合群体，亚群间遗传分化明显，基因交流较少。系统发生树表明，桂花栽培品种演化历程大致分为10个阶段，大部分银桂品种和四季桂品种最先形成，雄性丹桂品种最晚形成，其他品种处于演化的中间阶段。两性花品种比例随着演化水平的增高呈现逐渐降低的趋势，证明桂花的雄全异株性别系统是雌雄同花演化为雌雄异株的中间状态。此外，桂花花色连续变异可能受到多个CCDs家族同源基因突变的影响，而遗传漂变导致新等位基因丢失，这可能是野生桂花罕见橙红色个体的原因。
Bibliography:Sweet osmanthus(Osmanthus fragrans)is one of the top ten famous native horticultural plants in China.According to different flowering seasons,flower colors and inflorescence types,the cultivars are divided into four groups:O.fragrans Asiaticus Group,O.fragrans Albus Group,O.fragrans Luteus Group and O.fragrans Aurantiacus Group.Despite long-term cultivation of Osmanthus,little information was recorded on the formation of so many cultivars.O.fragrans Asiaticus Group was considered as the most primitive cultivars,and the ones with light color flowers formed earlier,then followed by deep color flowers.The cluster results based on various types of molecular markers were quite different from traditional classification system,indicating diversity of phenotypic traits might account for a tiny part of the whole genetic diversity of sweet osmanthus.However,at present,few studies can give evidence to further understand the evolution process of sweet osmanthus cultivars.In this study,to further understand the evolution
ISSN:1008-9209
DOI:10.3785/j.issn.1008-9209.2016.08.241