QTL analysis of resistance to bacterial wilt caused by Ralstonia solanacearum in potato

• Published in: Breeding Science Vol. 69; no. 4; pp. 592 - 600
• Main Authors: Habe, Ippei, Miyatake, Koji, Nunome, Tsukasa, Yamasaki, Masanori, Hayashi, Takeshi
• Format: Journal Article
• Language: English; Japanese
• Published: Japan Japanese Society of Breeding 01.01.2019; Japan Science and Technology Agency
• Subjects: 808 potato SNP array; Alleles; Bacteria; bacterial wilt; Chromosomes; Cloning; Gene mapping; Gene polymorphism; Genomes; In vitro methods and tests; Infinium 12; Inoculation; Markers; Nucleotides; Phenotypic variations; Polymorphism; potato; Potatoes; Quantitative trait loci; Ralstonia solanacearum; Research Paper; resistance QTLs; Single-nucleotide polymorphism; Soil microorganisms; Soil-borne diseases; Wilt; bacterial wilt; Infinium 12; Ralstonia solanacearum; resistance QTLs; 808 potato SNP array; potato
• ISSN: 1344-7610; 1347-3735
• DOI: 10.1270/jsbbs.19059

Ralstonia solanacearum causes bacterial wilt, a soil-borne disease and one of the most important maladies of potato and other Solanaceae crops. We analyzed the resistance of a potato clone to bacterial wilt by quantitative trait locus (QTL) analysis. A resistant diploid potato clone 10-03-30 was crossed with a susceptible diploid clone F1-1 to generate a diploid, two-way pseudo-testcross F1 population comprised of 94 genotypes. Dense linkage maps, containing 4,139 single nucleotide polymorphism markers with an average distance of 0.6 and 0.3 cM between markers, were constructed for both parents. The resistance level was evaluated by in vitro inoculation test with R. solanacearum (phylotype I/biovar 4/race 1). Five QTLs (qBWR-1 to -5) were identified on potato chromosomes 1, 3, 7, 10, and 11, and they explained 9.3–18.4% of the phenotypic variance. The resistant parent had resistant alleles in qBWR-2, qBWR-3, and qBWR-4 and susceptible alleles in qBWR-1 and qBWR-5. Accumulation of the resistant alleles in all five QTLs increased the level of resistance compared with that of the resistant parent. This is the first study to identify novel QTLs for bacterial wilt resistance in potato by using genome-wide markers.