Conferring DNA virus resistance with high specificity in plants using virus-inducible genome-editing system

• Published in: Genome Biology Vol. 19; no. 1; p. 197
• Main Authors: Ji, Xiang, Si, Xiaomin, Zhang, Yi, Zhang, Huawei, Zhang, Feng, Gao, Caixia
• Format: Journal Article
• Language: English
• Published: England BioMed Central 15.11.2018; BMC
• Subjects: Arabidopsis; Beet severe curly top virus; Bioinformatics; CRISPR; CRISPR-Cas Systems; CRISPR/Cas9 system; Deoxyribonucleic acid; Disease Resistance; DNA; Geminiviridae; Geminivirus; gene editing; Gene Editing - methods; Gene expression; genetically modified organisms; genome; Genomes; Immune system; Infections; Nicotiana; Nicotiana benthamiana; Off-target; Plant viruses; Short Report; Transgenic plants; Virology; Virus-inducible; Virus-resistance; Viruses; Geminivirus; Off-target; Virus-resistance; CRISPR/Cas9 system; Virus-inducible

The CRISPR/Cas9 system has recently been engineered to confer resistance to geminiviruses in plants. However, we show here that the usefulness of this antiviral strategy is undermined by off-target effects identified by deep sequencing in Arabidopsis. We construct two virus-inducible CRISPR/Cas9 vec...