Conferring DNA virus resistance with high specificity in plants using virus-inducible genome-editing system

The CRISPR/Cas9 system has recently been engineered to confer resistance to geminiviruses in plants. However, we show here that the usefulness of this antiviral strategy is undermined by off-target effects identified by deep sequencing in Arabidopsis. We construct two virus-inducible CRISPR/Cas9 vec...

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Published inGenome Biology Vol. 19; no. 1; p. 197
Main Authors Ji, Xiang, Si, Xiaomin, Zhang, Yi, Zhang, Huawei, Zhang, Feng, Gao, Caixia
Format Journal Article
LanguageEnglish
Published England BioMed Central 15.11.2018
BMC
Subjects
Arabidopsis
Beet severe curly top virus
Bioinformatics
CRISPR
CRISPR-Cas Systems
CRISPR/Cas9 system
Deoxyribonucleic acid
Disease Resistance
DNA
Geminiviridae
Geminivirus
gene editing
Gene Editing - methods
Gene expression
genetically modified organisms
genome
Genomes
Immune system
Infections
Nicotiana
Nicotiana benthamiana
Off-target
Plant viruses
Short Report
Transgenic plants
Virology
Virus-inducible
Virus-resistance
Viruses
Geminivirus
Off-target
Virus-resistance
CRISPR/Cas9 system
Virus-inducible
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Summary:The CRISPR/Cas9 system has recently been engineered to confer resistance to geminiviruses in plants. However, we show here that the usefulness of this antiviral strategy is undermined by off-target effects identified by deep sequencing in Arabidopsis. We construct two virus-inducible CRISPR/Cas9 vectors that efficiently inhibit beet severe curly top virus (BSCTV) accumulation in both transient assays (Nicotiana benthamiana) and transgenic lines (Arabidopsis). Deep sequencing detects no off-target effect in candidate sites of the transgenic Arabidopsis. This kind of virus-inducible genome-editing system should be widely applicable for generating virus-resistant plants without off-target costs.
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ISSN:1474-760X
1474-7596
1474-760X
DOI:10.1186/s13059-018-1580-4