Conferring DNA virus resistance with high specificity in plants using virus-inducible genome-editing system

The CRISPR/Cas9 system has recently been engineered to confer resistance to geminiviruses in plants. However, we show here that the usefulness of this antiviral strategy is undermined by off-target effects identified by deep sequencing in Arabidopsis. We construct two virus-inducible CRISPR/Cas9 vec...

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Published inGenome Biology Vol. 19; no. 1; p. 197
Main Authors Ji, Xiang, Si, Xiaomin, Zhang, Yi, Zhang, Huawei, Zhang, Feng, Gao, Caixia
Format Journal Article
LanguageEnglish
Published England BioMed Central 15.11.2018
BMC
Subjects
Arabidopsis
Beet severe curly top virus
Bioinformatics
CRISPR
CRISPR-Cas Systems
CRISPR/Cas9 system
Deoxyribonucleic acid
Disease Resistance
DNA
Geminiviridae
Geminivirus
gene editing
Gene Editing - methods
Gene expression
genetically modified organisms
genome
Genomes
Immune system
Infections
Nicotiana
Nicotiana benthamiana
Off-target
Plant viruses
Short Report
Transgenic plants
Virology
Virus-inducible
Virus-resistance
Viruses
Geminivirus
Off-target
Virus-resistance
CRISPR/Cas9 system
Virus-inducible
Online AccessGet full text

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Abstract The CRISPR/Cas9 system has recently been engineered to confer resistance to geminiviruses in plants. However, we show here that the usefulness of this antiviral strategy is undermined by off-target effects identified by deep sequencing in Arabidopsis. We construct two virus-inducible CRISPR/Cas9 vectors that efficiently inhibit beet severe curly top virus (BSCTV) accumulation in both transient assays (Nicotiana benthamiana) and transgenic lines (Arabidopsis). Deep sequencing detects no off-target effect in candidate sites of the transgenic Arabidopsis. This kind of virus-inducible genome-editing system should be widely applicable for generating virus-resistant plants without off-target costs.
AbstractList The CRISPR/Cas9 system has recently been engineered to confer resistance to geminiviruses in plants. However, we show here that the usefulness of this antiviral strategy is undermined by off-target effects identified by deep sequencing in Arabidopsis. We construct two virus-inducible CRISPR/Cas9 vectors that efficiently inhibit beet severe curly top virus (BSCTV) accumulation in both transient assays (Nicotiana benthamiana) and transgenic lines (Arabidopsis). Deep sequencing detects no off-target effect in candidate sites of the transgenic Arabidopsis. This kind of virus-inducible genome-editing system should be widely applicable for generating virus-resistant plants without off-target costs.The CRISPR/Cas9 system has recently been engineered to confer resistance to geminiviruses in plants. However, we show here that the usefulness of this antiviral strategy is undermined by off-target effects identified by deep sequencing in Arabidopsis. We construct two virus-inducible CRISPR/Cas9 vectors that efficiently inhibit beet severe curly top virus (BSCTV) accumulation in both transient assays (Nicotiana benthamiana) and transgenic lines (Arabidopsis). Deep sequencing detects no off-target effect in candidate sites of the transgenic Arabidopsis. This kind of virus-inducible genome-editing system should be widely applicable for generating virus-resistant plants without off-target costs.
Abstract The CRISPR/Cas9 system has recently been engineered to confer resistance to geminiviruses in plants. However, we show here that the usefulness of this antiviral strategy is undermined by off-target effects identified by deep sequencing in Arabidopsis. We construct two virus-inducible CRISPR/Cas9 vectors that efficiently inhibit beet severe curly top virus (BSCTV) accumulation in both transient assays (Nicotiana benthamiana) and transgenic lines (Arabidopsis). Deep sequencing detects no off-target effect in candidate sites of the transgenic Arabidopsis. This kind of virus-inducible genome-editing system should be widely applicable for generating virus-resistant plants without off-target costs.
The CRISPR/Cas9 system has recently been engineered to confer resistance to geminiviruses in plants. However, we show here that the usefulness of this antiviral strategy is undermined by off-target effects identified by deep sequencing in Arabidopsis. We construct two virus-inducible CRISPR/Cas9 vectors that efficiently inhibit beet severe curly top virus (BSCTV) accumulation in both transient assays (Nicotiana benthamiana) and transgenic lines (Arabidopsis). Deep sequencing detects no off-target effect in candidate sites of the transgenic Arabidopsis. This kind of virus-inducible genome-editing system should be widely applicable for generating virus-resistant plants without off-target costs.
The CRISPR/Cas9 system has recently been engineered to confer resistance to geminiviruses in plants. However, we show here that the usefulness of this antiviral strategy is undermined by off-target effects identified by deep sequencing in Arabidopsis . We construct two virus-inducible CRISPR/Cas9 vectors that efficiently inhibit beet severe curly top virus (BSCTV) accumulation in both transient assays ( Nicotiana benthamiana ) and transgenic lines ( Arabidopsis ). Deep sequencing detects no off-target effect in candidate sites of the transgenic Arabidopsis . This kind of virus-inducible genome-editing system should be widely applicable for generating virus-resistant plants without off-target costs.
ArticleNumber 197
Author Zhang, Yi
Si, Xiaomin
Gao, Caixia
Ji, Xiang
Zhang, Huawei
Zhang, Feng
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Cites_doi 10.1016/j.virol.2016.04.033
10.1006/viro.1996.0292
10.1016/j.plantsci.2008.08.011
10.1093/bioinformatics/btu048
10.1038/nmeth.1318
10.1038/nbt.2673
10.1038/nchembio.1793
10.1186/s13059-018-1580-4
10.1186/s13059-015-0829-4
10.1111/pbi.12881
10.1038/nbt.2647
10.1038/nature11629
10.1038/nplants.2015.146
10.1016/S1016-8478(23)13974-4
10.1186/s13059-015-0799-6
10.1002/j.1460-2075.1987.tb02730.x
10.1007/s11103-018-0709-x
10.1038/nplants.2015.144
10.1038/nplants.2015.145
10.1186/s12870-014-0327-y
10.1038/srep26912
10.1038/nrg.2016.28
10.1038/nmeth.4368
10.1016/j.tplants.2016.01.023
10.1038/nbt.2623
10.1093/bioinformatics/btw561
10.1038/nprot.2014.157
10.1038/nrmicro3117
10.1046/j.1365-313x.1998.00343.x
10.1146/annurev-phyto-072910-095235
10.1186/s13059-017-1381-1
10.1038/ng.3200
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Issue 1
Keywords Geminivirus
Off-target
Virus-resistance
CRISPR/Cas9 system
Virus-inducible
Language English
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References T Zhang (1580_CR26) 2018; 16
SQ Tsai (1580_CR12) 2016; 17
HL Xing (1580_CR31) 2014; 14
Q Shan (1580_CR32) 2014; 9
X Ji (1580_CR6) 2015; 1
RA Jefferson (1580_CR30) 1987; 6
1580_CR34
1580_CR33
H Liu (1580_CR8) 2018; 2
JY Hur (1580_CR20) 2008; 26
KM Davis (1580_CR18) 2015; 11
NJ Baltes (1580_CR5) 2015; 1
V Pattanayak (1580_CR13) 2013; 31
Y Fu (1580_CR14) 2013; 31
PD Hsu (1580_CR15) 2013; 31
RE Handsaker (1580_CR24) 2015; 47
Z Ali (1580_CR4) 2015; 16
Z Ali (1580_CR7) 2016; 6
BK Borah (1580_CR22) 2016; 495
R Aman (1580_CR25) 2018; 19
J Navas-Castillo (1580_CR2) 2011; 49
DN Shepherd (1580_CR3) 2009; 176
D Zhang (1580_CR11) 2015; 1
L Hanley-Bowdoin (1580_CR1) 2013; 11
A Abyzov (1580_CR23) 2012; 492
DG Gibson (1580_CR29) 2009; 6
J Park (1580_CR28) 2017; 33
A Chaparro-Garcia (1580_CR9) 2015; 16
JC Rose (1580_CR19) 2017; 14
SJ Clough (1580_CR27) 1998; 16
SS Zaidi (1580_CR10) 2016; 21
Q Zhang (1580_CR16) 2018; 96
S Bae (1580_CR17) 2014; 30
Y Hong (1580_CR21) 1996; 220
References_xml – volume: 495
  start-page: 101
  year: 2016
  ident: 1580_CR22
  publication-title: Virology
  doi: 10.1016/j.virol.2016.04.033
– volume: 220
  start-page: 119
  year: 1996
  ident: 1580_CR21
  publication-title: Virology
  doi: 10.1006/viro.1996.0292
– volume: 176
  start-page: 1
  year: 2009
  ident: 1580_CR3
  publication-title: Plant Sci
  doi: 10.1016/j.plantsci.2008.08.011
– volume: 30
  start-page: 1473
  year: 2014
  ident: 1580_CR17
  publication-title: Bioinformatics
  doi: 10.1093/bioinformatics/btu048
– volume: 6
  start-page: 343
  year: 2009
  ident: 1580_CR29
  publication-title: Nat Methods
  doi: 10.1038/nmeth.1318
– volume: 31
  start-page: 839
  year: 2013
  ident: 1580_CR13
  publication-title: Nat Biotechnol
  doi: 10.1038/nbt.2673
– volume: 11
  start-page: 316
  year: 2015
  ident: 1580_CR18
  publication-title: Nat Chem Biol
  doi: 10.1038/nchembio.1793
– ident: 1580_CR34
  doi: 10.1186/s13059-018-1580-4
– volume: 16
  start-page: 254
  year: 2015
  ident: 1580_CR9
  publication-title: Genome Biol
  doi: 10.1186/s13059-015-0829-4
– volume: 16
  start-page: 1415
  year: 2018
  ident: 1580_CR26
  publication-title: Plant Biotechnol J
  doi: 10.1111/pbi.12881
– volume: 2
  start-page: e00047
  year: 2018
  ident: 1580_CR8
  publication-title: Direct
– volume: 31
  start-page: 827
  year: 2013
  ident: 1580_CR15
  publication-title: Nat Biotechnol
  doi: 10.1038/nbt.2647
– volume: 492
  start-page: 438
  year: 2012
  ident: 1580_CR23
  publication-title: Nature
  doi: 10.1038/nature11629
– volume: 1
  year: 2015
  ident: 1580_CR11
  publication-title: Nat Plants.
  doi: 10.1038/nplants.2015.146
– volume: 26
  start-page: 131
  year: 2008
  ident: 1580_CR20
  publication-title: Molecules Cells
  doi: 10.1016/S1016-8478(23)13974-4
– volume: 16
  start-page: 238
  year: 2015
  ident: 1580_CR4
  publication-title: Genome Biol
  doi: 10.1186/s13059-015-0799-6
– volume: 6
  start-page: 3901
  year: 1987
  ident: 1580_CR30
  publication-title: EMBO J
  doi: 10.1002/j.1460-2075.1987.tb02730.x
– volume: 96
  start-page: 445
  year: 2018
  ident: 1580_CR16
  publication-title: Plant Mol Biol
  doi: 10.1007/s11103-018-0709-x
– volume: 1
  year: 2015
  ident: 1580_CR6
  publication-title: Nat Plants.
  doi: 10.1038/nplants.2015.144
– volume: 1
  year: 2015
  ident: 1580_CR5
  publication-title: Nat Plants
  doi: 10.1038/nplants.2015.145
– volume: 14
  start-page: 327
  year: 2014
  ident: 1580_CR31
  publication-title: BMC Plant Biol
  doi: 10.1186/s12870-014-0327-y
– volume: 6
  start-page: 26912
  year: 2016
  ident: 1580_CR7
  publication-title: Sci Rep
  doi: 10.1038/srep26912
– volume: 17
  start-page: 300
  year: 2016
  ident: 1580_CR12
  publication-title: Nat Rev Genet
  doi: 10.1038/nrg.2016.28
– volume: 14
  start-page: 891
  year: 2017
  ident: 1580_CR19
  publication-title: Nat Methods
  doi: 10.1038/nmeth.4368
– volume: 21
  start-page: 279
  year: 2016
  ident: 1580_CR10
  publication-title: Trends Plant Sci
  doi: 10.1016/j.tplants.2016.01.023
– volume: 31
  start-page: 822
  year: 2013
  ident: 1580_CR14
  publication-title: Nat Biotechnol
  doi: 10.1038/nbt.2623
– volume: 33
  start-page: 286
  year: 2017
  ident: 1580_CR28
  publication-title: Bioinformatics
  doi: 10.1093/bioinformatics/btw561
– ident: 1580_CR33
  doi: 10.1186/s13059-018-1580-4
– volume: 9
  start-page: 2395
  year: 2014
  ident: 1580_CR32
  publication-title: Nat Protoc
  doi: 10.1038/nprot.2014.157
– volume: 11
  start-page: 777
  year: 2013
  ident: 1580_CR1
  publication-title: Nat Rev Microbiol
  doi: 10.1038/nrmicro3117
– volume: 16
  start-page: 735
  year: 1998
  ident: 1580_CR27
  publication-title: Plant J
  doi: 10.1046/j.1365-313x.1998.00343.x
– volume: 49
  start-page: 219
  year: 2011
  ident: 1580_CR2
  publication-title: Annu Rev Phytopathol
  doi: 10.1146/annurev-phyto-072910-095235
– volume: 19
  start-page: 1
  year: 2018
  ident: 1580_CR25
  publication-title: Genome Biol
  doi: 10.1186/s13059-017-1381-1
– volume: 47
  start-page: 296
  year: 2015
  ident: 1580_CR24
  publication-title: Nat Genetics
  doi: 10.1038/ng.3200
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Snippet The CRISPR/Cas9 system has recently been engineered to confer resistance to geminiviruses in plants. However, we show here that the usefulness of this...
Abstract The CRISPR/Cas9 system has recently been engineered to confer resistance to geminiviruses in plants. However, we show here that the usefulness of this...
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SubjectTerms Arabidopsis
Beet severe curly top virus
Bioinformatics
CRISPR
CRISPR-Cas Systems
CRISPR/Cas9 system
Deoxyribonucleic acid
Disease Resistance
DNA
Geminiviridae
Geminivirus
gene editing
Gene Editing - methods
Gene expression
genetically modified organisms
genome
Genomes
Immune system
Infections
Nicotiana
Nicotiana benthamiana
Off-target
Plant viruses
Short Report
Transgenic plants
Virology
Virus-inducible
Virus-resistance
Viruses
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Title Conferring DNA virus resistance with high specificity in plants using virus-inducible genome-editing system
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