Reconstruction of the cell entry pathway of an extinct virus

• Published in: PLoS pathogens Vol. 14; no. 8; p. e1007123
• Main Authors: Robinson-McCarthy, Lindsey R., McCarthy, Kevin R., Raaben, Matthijs, Piccinotti, Silvia, Nieuwenhuis, Joppe, Stubbs, Sarah H., Bakkers, Mark J. G., Whelan, Sean P. J.
• Format: Journal Article
• Language: English
• Published: United States Public Library of Science 01.08.2018; Public Library of Science (PLoS)
• Subjects: Acids; Anticoagulants; Binding sites; Biochemistry; Biology and life sciences; Biosynthesis; Cancer; Cell surface; CRISPR; Endangered & extinct species; Endogenous retroviruses; Endogenous Retroviruses - physiology; Extinction; Fusion protein; Genes; Genetic aspects; Genetic screening; Genomes; Glycoproteins; Heparan sulfate; Heparin; Heparitin Sulfate - metabolism; History; Humans; Infections; Infectious diseases; Leukemia; Medical schools; Medicine and Health Sciences; Mutation; Organic chemistry; pH effects; Physical Sciences; Physiological aspects; Pretreatment; Proteins; Research and Analysis Methods; Retroviruses; RNA polymerase; Stem cells; Stomatitis; Sulfates; Viral Envelope Proteins - metabolism; Viral Tropism - physiology; Virions; Virology; Virus attachment; Virus Internalization; Virus replication; Viruses; United States; Netherlands; United States > US; Massachusetts
• ISSN: 1553-7374; 1553-7366; 1553-7374
• DOI: 10.1371/journal.ppat.1007123

Endogenous retroviruses (ERVs), remnants of ancient germline infections, comprise 8% of the human genome. The most recently integrated includes human ERV-K (HERV-K) where several envelope (env) sequences remain intact. Viral pseudotypes decorated with one of those Envs are infectious. Using a recombinant vesicular stomatitis virus encoding HERV-K Env as its sole attachment and fusion protein (VSV-HERVK) we conducted a genome-wide haploid genetic screen to interrogate the host requirements for infection. This screen identified 11 genes involved in heparan sulfate biosynthesis. Genetic inhibition or chemical removal of heparan sulfate and addition of excess soluble heparan sulfate inhibit infection. Direct binding of heparin to soluble HERV-K Env and purified VSV-HERVK defines it as critical for viral attachment. Cell surface bound VSV-HERVK particles are triggered to infect on exposure to acidic pH, whereas acid pH pretreatment of virions blocks infection. Testing of additional endogenous HERV-K env sequences reveals they bind heparin and mediate acid pH triggered fusion. This work reconstructs and defines key steps in the infectious entry pathway of an extinct virus.