CDetection: CRISPR-Cas12b-based DNA detection with sub-attomolar sensitivity and single-base specificity
CRISPR-based nucleic acid detection methods are reported to facilitate rapid and sensitive DNA detection. However, precise DNA detection at the single-base resolution and its wide applications including high-fidelity SNP genotyping remain to be explored. Here we develop a Cas12b-mediated DNA detecti...
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| Published in | Genome Biology Vol. 20; no. 1; p. 132 |
|---|---|
| Main Authors | , , , , , , , |
| Format | Journal Article |
| Language | English |
| Published |
England
BioMed Central
01.07.2019
BMC |
| Subjects | |
| Online Access | Get full text |
| ISSN | 1474-760X 1474-7596 1474-760X |
| DOI | 10.1186/s13059-019-1742-z |
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| Abstract | CRISPR-based nucleic acid detection methods are reported to facilitate rapid and sensitive DNA detection. However, precise DNA detection at the single-base resolution and its wide applications including high-fidelity SNP genotyping remain to be explored. Here we develop a Cas12b-mediated DNA detection (CDetection) strategy, which shows higher sensitivity on examined targets compared with the previously reported Cas12a-based detection platform. Moreover, we show that CDetection can distinguish differences at the single-base level upon combining the optimized tuned guide RNA (tgRNA). Therefore, our findings highlight the high sensitivity and accuracy of CDetection, which provides an efficient and highly practical platform for DNA detection. |
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| AbstractList | Abstract CRISPR-based nucleic acid detection methods are reported to facilitate rapid and sensitive DNA detection. However, precise DNA detection at the single-base resolution and its wide applications including high-fidelity SNP genotyping remain to be explored. Here we develop a Cas12b-mediated DNA detection (CDetection) strategy, which shows higher sensitivity on examined targets compared with the previously reported Cas12a-based detection platform. Moreover, we show that CDetection can distinguish differences at the single-base level upon combining the optimized tuned guide RNA (tgRNA). Therefore, our findings highlight the high sensitivity and accuracy of CDetection, which provides an efficient and highly practical platform for DNA detection. CRISPR-based nucleic acid detection methods are reported to facilitate rapid and sensitive DNA detection. However, precise DNA detection at the single-base resolution and its wide applications including high-fidelity SNP genotyping remain to be explored. Here we develop a Cas12b-mediated DNA detection (CDetection) strategy, which shows higher sensitivity on examined targets compared with the previously reported Cas12a-based detection platform. Moreover, we show that CDetection can distinguish differences at the single-base level upon combining the optimized tuned guide RNA (tgRNA). Therefore, our findings highlight the high sensitivity and accuracy of CDetection, which provides an efficient and highly practical platform for DNA detection.CRISPR-based nucleic acid detection methods are reported to facilitate rapid and sensitive DNA detection. However, precise DNA detection at the single-base resolution and its wide applications including high-fidelity SNP genotyping remain to be explored. Here we develop a Cas12b-mediated DNA detection (CDetection) strategy, which shows higher sensitivity on examined targets compared with the previously reported Cas12a-based detection platform. Moreover, we show that CDetection can distinguish differences at the single-base level upon combining the optimized tuned guide RNA (tgRNA). Therefore, our findings highlight the high sensitivity and accuracy of CDetection, which provides an efficient and highly practical platform for DNA detection. CRISPR-based nucleic acid detection methods are reported to facilitate rapid and sensitive DNA detection. However, precise DNA detection at the single-base resolution and its wide applications including high-fidelity SNP genotyping remain to be explored. Here we develop a Cas12b-mediated DNA detection (CDetection) strategy, which shows higher sensitivity on examined targets compared with the previously reported Cas12a-based detection platform. Moreover, we show that CDetection can distinguish differences at the single-base level upon combining the optimized tuned guide RNA (tgRNA). Therefore, our findings highlight the high sensitivity and accuracy of CDetection, which provides an efficient and highly practical platform for DNA detection. |
| ArticleNumber | 132 |
| Author | Cui, Tongtong Zhou, Qi Xu, Kai Li, Wei Teng, Fei Wang, Xin-Ge Guo, Lu Gao, Qingqin |
| Author_xml | – sequence: 1 givenname: Fei surname: Teng fullname: Teng, Fei – sequence: 2 givenname: Lu surname: Guo fullname: Guo, Lu – sequence: 3 givenname: Tongtong surname: Cui fullname: Cui, Tongtong – sequence: 4 givenname: Xin-Ge surname: Wang fullname: Wang, Xin-Ge – sequence: 5 givenname: Kai surname: Xu fullname: Xu, Kai – sequence: 6 givenname: Qingqin surname: Gao fullname: Gao, Qingqin – sequence: 7 givenname: Qi surname: Zhou fullname: Zhou, Qi – sequence: 8 givenname: Wei surname: Li fullname: Li, Wei |
| BackLink | https://www.ncbi.nlm.nih.gov/pubmed/31262344$$D View this record in MEDLINE/PubMed |
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| Snippet | CRISPR-based nucleic acid detection methods are reported to facilitate rapid and sensitive DNA detection. However, precise DNA detection at the single-base... Abstract CRISPR-based nucleic acid detection methods are reported to facilitate rapid and sensitive DNA detection. However, precise DNA detection at the... |
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| SubjectTerms | CRISPR CRISPR-Cas Systems Deoxyribonucleic acid DNA DNA - analysis Enzymes Escherichia coli Genetic Techniques Genetic Testing - methods genome Genomes Genotyping Humans Ribonucleic acid RNA Sensitivity and Specificity Short Report Single-nucleotide polymorphism Zika virus |
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| Title | CDetection: CRISPR-Cas12b-based DNA detection with sub-attomolar sensitivity and single-base specificity |
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