CDetection: CRISPR-Cas12b-based DNA detection with sub-attomolar sensitivity and single-base specificity

CRISPR-based nucleic acid detection methods are reported to facilitate rapid and sensitive DNA detection. However, precise DNA detection at the single-base resolution and its wide applications including high-fidelity SNP genotyping remain to be explored. Here we develop a Cas12b-mediated DNA detecti...

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Published inGenome Biology Vol. 20; no. 1; p. 132
Main Authors Teng, Fei, Guo, Lu, Cui, Tongtong, Wang, Xin-Ge, Xu, Kai, Gao, Qingqin, Zhou, Qi, Li, Wei
Format Journal Article
LanguageEnglish
Published England BioMed Central 01.07.2019
BMC
Subjects
CRISPR
CRISPR-Cas Systems
Deoxyribonucleic acid
DNA
DNA - analysis
Enzymes
Escherichia coli
Genetic Techniques
Genetic Testing - methods
genome
Genomes
Genotyping
Humans
Ribonucleic acid
RNA
Sensitivity and Specificity
Short Report
Single-nucleotide polymorphism
Zika virus
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Summary:CRISPR-based nucleic acid detection methods are reported to facilitate rapid and sensitive DNA detection. However, precise DNA detection at the single-base resolution and its wide applications including high-fidelity SNP genotyping remain to be explored. Here we develop a Cas12b-mediated DNA detection (CDetection) strategy, which shows higher sensitivity on examined targets compared with the previously reported Cas12a-based detection platform. Moreover, we show that CDetection can distinguish differences at the single-base level upon combining the optimized tuned guide RNA (tgRNA). Therefore, our findings highlight the high sensitivity and accuracy of CDetection, which provides an efficient and highly practical platform for DNA detection.
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ISSN:1474-760X
1474-7596
1474-760X
DOI:10.1186/s13059-019-1742-z