Combination of ELISA screening and seroneutralisation tests to expedite Zika virus seroprevalence studies

• Published in: Virology journal Vol. 15; no. 1; p. 192
• Main Authors: Nurtop, Elif, Villarroel, Paola Mariela Saba, Pastorino, Boris, Ninove, Laetitia, Drexler, Jan Felix, Roca, Yelin, Gake, Bouba, Dubot-Peres, Audrey, Grard, Gilda, Peyrefitte, Christophe, Priet, Stéphane, de Lamballerie, Xavier, Gallian, Pierre
• Format: Journal Article
• Language: English
• Published: England BioMed Central Ltd 27.12.2018; BioMed Central; BMC
• Subjects: Analysis; Antibodies, Neutralizing; Antibodies, Neutralizing - blood; Antibodies, Viral; Antibodies, Viral - blood; Blood & organ donations; Blood donors; cost effectiveness; Cytopathogenic Effect, Viral; dengue; Dengue fever; Diagnosis; Encephalitis; Enzyme-Linked Immunosorbent Assay; Enzyme-Linked Immunosorbent Assay - methods; Guillain-Barre syndrome; Humans; Immunoglobulin G; Immunoglobulin G - blood; Infections; Laboratories; Life Sciences; Martinique; Martinique - epidemiology; Mass Screening; Mass Screening - methods; Microbiology and Parasitology; Microcephaly; Microscopy; Neutralization Tests; Neutralization Tests - methods; NS1 protein; Prevalence studies (Epidemiology); Proteins; Santé publique et épidémiologie; screening; Sensitivity and Specificity; Seroepidemiologic Studies; Seroepidemiology; Serologic Tests; Serologic Tests - methods; serological surveys; seroprevalence; Short Report; Studies; Virology; Virus neutralization test; Zika Virus; Zika Virus - immunology; Zika Virus Infection; Zika Virus Infection - diagnosis; Zika Virus Infection - epidemiology; Martinique; United States > US; French Polynesia; Seroepidemiology; Zika virus; Virus neutralization test
• ISSN: 1743-422X; 1743-422X
• DOI: 10.1186/s12985-018-1105-5

Here we propose a strategy allowing implementing efficient and practicable large-scale seroepidemiological studies for Zika Virus (ZIKV). It combines screening by a commercial NS1 protein-based Zika IgG ELISA, and confirmation by a cytopathic effect-based virus neutralization test (CPE-based VNT). In post-epidemic samples from Martinique Island blood donors (a population with a dengue seroprevalence above 90%), this strategy allowed reaching specificity and sensitivity values over 98%. The CPE-based VNT consists of recording CPE directly under the optical microscope, which is easy to identify with ZIKV strain H/PF/2013 at day 5 pi. Overall, considered that CPE-based VNT is cost effective and widely automatable, the NS1 protein-based Zika IgG ELISA+CPE-based VNT combination strategy represents a convenient tool to expedite ZIKV seroprevalence studies.