Augmented Activity of the Pelvic Nerve Afferent Mediated by TRP Channels in Dextran Sulfate Sodium (DSS)-Induced Colitis of Rats

• Published in: Journal of Veterinary Medical Science Vol. 74; no. 8; pp. 1007 - 1013
• Main Authors: MAKIMURA, Yukitoshi, ITO, Koichi, KUWAHARA, Masayoshi, TSUBONE, Hirokazu
• Format: Journal Article
• Language: English
• Published: Japan JAPANESE SOCIETY OF VETERINARY SCIENCE 01.08.2012; Japan Science and Technology Agency
• Subjects: Acetic Acid - administration & dosage; Acetic Acid - toxicity; Animals; C-fiber; Capsaicin - administration & dosage; Capsaicin - toxicity; colitis; Colitis - chemically induced; Colitis - metabolism; Dextran Sulfate - toxicity; Electrophysiological Phenomena; Male; Neurons, Afferent - drug effects; nociception; Pain - chemically induced; pelvic nerve; Rats; Rats, Inbred WKY; Ruthenium Red - pharmacology; Transient Receptor Potential Channels - physiology
• ISSN: 0916-7250; 1347-7439
• DOI: 10.1292/jvms.11-0547

Enteritis has been recognized as a major symptom in domestic animals and human patients suffering from feed and food poisonings. The aim of the present study was to clarify the excitatory mechanism of the pelvic nerve afferent which may influence the occurrence of enteritis in response to nociceptive chemical stimuli of the colon in normal and abnormal rats with colitis induced by dextran sulfate sodium (DSS). The pelvic nerve afferent activity was markedly increased by colonic instillation of solution (0.5 ml) of acetic acid (5–25%) and capsaicin (100μg/ml). The nerve activity was augmented by colonic instillation of capsaicin to a greater extent in rats with DSS-induced colitis than in normal control rats. This augmented activity by capsaicin was more prominent at one day (DSS-1) than at 8 day (DSS-8) after the administration of DSS. The increased nerve activity caused by capsaicin in DSS-1 and DSS-8 was significantly inhibited by pretreatment with ruthenium red, which is a nonselective inhibitor of TRP channels of unmyelinated C-fibers (nociceptors). In conclusion, it was elucidated that the nociceptive function of the pelvic nerve was largely elevated at one day after DSS-induced colitis and such increased function was mostly mediated by TRP channels.