Validation of the usefulness of 26S rDNA D1/D2, internal transcribed spacer, and intergenic spacer 1 for molecular epidemiological analysis of Macrorhabdus ornithogaster

• Published in: Journal of Veterinary Medical Science Vol. 84; no. 2; pp. 244 - 250
• Main Authors: KOJIMA, Atsushi, OSAWA, Nanako, OBA, Mami, KATAYAMA, Yukie, OMATSU, Tsutomu, MIZUTANI, Tetsuya
• Format: Journal Article
• Language: English
• Published: Japan JAPANESE SOCIETY OF VETERINARY SCIENCE 2022; Japan Science and Technology Agency; The Japanese Society of Veterinary Science
• Subjects: 26S rDNA D1/D2; Animals; Bacteriology; DNA, Intergenic - genetics; DNA, Ribosomal; DNA, Ribosomal Spacer - genetics; Epidemiology; Genetic analysis; Genetic diversity; genotyping; intergenic space 1; internal transcribed spacer; Macrorhabdus ornithogaster; Phylogeny; Polymerase chain reaction; Ribosomal DNA; Saccharomycetales - genetics; Spacer; 26S rDNA D1/D2; intergenic space 1; Macrorhabdus ornithogaster; genotyping; internal transcribed spacer
• ISSN: 0916-7250; 1347-7439; 1347-7439
• DOI: 10.1292/jvms.21-0576

Macrorhabdus ornithogaster (MO) is an infectious fungus that causes gastric damage in birds. In this study, we established nested and seminested polymerase chain reaction (PCR) methods that specifically amplify the domain D1/D2 region (D1/D2) of 26S ribosomal DNA (rDNA), internal transcribed spacer (ITS) of rDNA, and intergenic spacer (IGS) 1 region from avian feces. Phylogenetic analysis of MO collected from Japanese pet birds showed little genetic variation; analysis based on these regions did not distinguish between host species order, differences in MO shape, or host gastrointestinal symptoms. These regions were found to be unsuitable for molecular epidemiological studies of MO and further investigation into other genetic regions is required.