Osa-miR7695 enhances transcriptional priming in defense responses against the rice blast fungus

• Published in: BMC plant biology Vol. 19; no. 1; p. 563
• Main Authors: Sánchez-Sanuy, Ferran, Peris-Peris, Cristina, Tomiyama, Shiho, Okada, Kazunori, Hsing, Yue-Ie, San Segundo, Blanca, Campo, Sonia
• Format: Journal Article
• Language: English
• Published: England BioMed Central Ltd 18.12.2019; BioMed Central; BMC
• Subjects: Alternative splicing; Analysis; Appressoria; Biochemistry; Blast; Defense; Deoxyribonucleic acid; Disease Resistance - genetics; Diterpenes; DNA; Eukaryotes; Explosions; Fungi; Gene expression; Gene Expression Regulation, Plant; Genes; Genetic aspects; Genomics; Health aspects; Homeostasis; Immunity; Infection; Infections; Iron; Iron industry; Macrophages; Magnaporthe - physiology; Magnaporthe oryzae; Microbial drug resistance; MicroRNA; MicroRNAs; MicroRNAs - genetics; MicroRNAs - metabolism; miR7695; miRNA; Oryza - genetics; Oryza - immunology; Pathogenesis; Pathogens; Phytoalexins; Plant Diseases - immunology; Plant growth; Plants (botany); Post-transcription; Priming; Proteins; Regulatory mechanisms (biology); Resistance factors; Rice; Rice blast; RNA; RNA, Plant - genetics; RNA, Plant - metabolism; Transcription (Genetics); miR7695; Oryza sativa; Phytoalexins; Transcriptomics; Magnaporthe oryzae; microRNA; Iron; Blast; Defense; Rice
• ISSN: 1471-2229; 1471-2229
• DOI: 10.1186/s12870-019-2156-5

MicroRNAs (miRNAs) are small non-coding RNAs that regulate gene expression at the post-transcriptional level in eukaryotes. In rice, MIR7695 expression is regulated by infection with the rice blast fungus Magnaporthe oryzae with subsequent down-regulation of an alternatively spliced transcript of natural resistance-associated macrophage protein 6 (OsNramp6). NRAMP6 functions as an iron transporter in rice. Rice plants grown under high iron supply showed blast resistance, which supports that iron is a factor in controlling blast resistance. During pathogen infection, iron accumulated in the vicinity of M. oryzae appressoria, the sites of pathogen entry, and in cells surrounding infected regions of the rice leaf. Activation-tagged MIR7695 rice plants (MIR7695-Ac) exhibited enhanced iron accumulation and resistance to M. oryzae infection. RNA-seq analysis revealed that blast resistance in MIR7695-Ac plants was associated with strong induction of defense-related genes, including pathogenesis-related and diterpenoid biosynthetic genes. Levels of phytoalexins during pathogen infection were higher in MIR7695-Ac than wild-type plants. Early phytoalexin biosynthetic genes, OsCPS2 and OsCPS4, were also highly upregulated in wild-type rice plants grown under high iron supply. Our data support a positive role of miR7695 in regulating rice immunity that further underpin links between defense and iron signaling in rice. These findings provides a basis to better understand regulatory mechanisms involved in rice immunity in which miR7695 participates which has a great potential for the development of strategies to improve blast resistance in rice.