Intracellular stimulation of an identified neuron evokes cardioacceleratory peptide release

• Published in: Science (American Association for the Advancement of Science) Vol. 228; no. 4702; pp. 1013 - 1015
• Main Authors: Tublitz, N.J, Truman, J.W
• Format: Journal Article
• Language: English
• Published: Washington, DC The American Association for the Advancement of Science 24.05.1985; American Association for the Advancement of Science
• Subjects: Abdominal ganglia; Action Potentials; Animals; Bioassay; Biochemistry. Physiology. Immunology; BIOLOGIA; Biological and medical sciences; Biological Assay; BIOLOGIE; BIOLOGY; Central nervous system; Electric Stimulation; Electrical stimulation; ESTIMULO; Fundamental and applied biological sciences. Psychology; Ganglia; Heart rate; Heart Rate - drug effects; Insect Hormones - metabolism; Insecta; Invertebrates; Lepidoptera - metabolism; MANDUCA; MANDUCA SEXTA; Moths - metabolism; Moths - physiology; NERF; Nerve Tissue Proteins - metabolism; Nerve Tissue Proteins - pharmacology; NERVES; NERVIOS; Neuroendocrinology; Neurons; Neurons - metabolism; Neuropeptides; PEPTIDE; PEPTIDES; PEPTIDOS; Physiological aspects; Physiology. Development; Pronase - pharmacology; Sphingidae; STIMULATION; Synapses - physiology; Heart rate; Neuron; Abdominal ganglion; Arthropoda; Insecta; Lepidoptera; Manduca sexta; Nervous system; Invertebrata; Sphingidae; Neuropeptide; Release
• ISSN: 0036-8075; 1095-9203
• DOI: 10.1126/science.4001929

The central nervous system of the tobacco hawkmoth, Manduca sexta, is known to contain two cardioacceleratory peptides (CAP's), both of which function in vivo as cardioregulatory neurohormones. Intracellular electrical stimulation of a single abdominal ganglion neuron evokes the release of CAP-like bioactivity. This stimulation-evoked bioactivity is destroyed by prior treatment with protease. The possibility that intracellular stimulation of a CAP-containing neuron synaptically activated additional spiking neurons is eliminated.