Screening of CXC chemokines in the microenvironment of ovarian cancer and the biological function of CXCL10

• Published in: World journal of surgical oncology Vol. 19; no. 1; p. 329
• Main Authors: Li, Weiyuan, Ma, Ji-Ao, Sheng, Xun, Xiao, Chunjie
• Format: Journal Article
• Language: English
• Published: England BioMed Central Ltd 18.11.2021; BioMed Central; BMC
• Subjects: Ability tests; Angiogenesis; Apoptosis; Assaying; Bioinformatics; Biological markers; Biomarkers; Cancer; Carcinoma, Ovarian Epithelial; Cell culture; Chemokine CXCL10; Chemokines; Chemokines, CXC - genetics; CXC chemokines; CXCL10 protein; CXCL11 protein; CXCL13 protein; Cytotoxicity; Development and progression; Early Detection of Cancer; Female; Gene expression; Genomics; Health aspects; Humans; Immune infiltration; Immune system; Immunoblotting; Immunotherapy; Lymphocytes; Lymphocytes T; Medical prognosis; Metastases; Microenvironments; Molecular biology; Ovarian cancer; Ovarian Neoplasms; Physiological aspects; Prognosis; Proteins; Software packages; Transcription factors; Tumor Microenvironment; Tumors; China; United States > US; Angiogenesis; Bioinformatics; Chemokines; Immune infiltration; Ovarian cancer
• ISSN: 1477-7819; 1477-7819
• DOI: 10.1186/s12957-021-02440-x

This study aims to screen and identify the biological functions and prognostic value of CXC chemokines in ovarian cancer (OC) through bioinformatics and molecular biology methods, and to provide data support for the selection of biomarkers and prognostic analysis of OC. In this study, GEO, ONCOMINE, GEPIA, cBioPortal, GeneMANIA, Metascape, STRING, TRRUST, and TIMER databases were used to study CXC chemokines. Angiogenesis and T cell killing assay were used to detect the effect of CXCL10 on tumor cell immunity and angiogenesis. Real-time quantitative PCR (qRT-PCR), immunoblotting, and ectopic tumor formation experiments were used to verify the effect of CXCL10 on ovarian cancer tumors. We found that CXCL1, CXCL10, CXCL11, CXCL13, and CXCL14 were significantly upregulated in OC samples compared with normal tissues. Our data showed that there was a relationship between the expression of CXC chemokines and the infiltration of six types of immune cells significant correlation. In vitro assay confirmed that overexpression of CXCL10 could enhance the killing effect of T cells and inhibit angiogenesis. Further in vivo assay had shown that CXCL10 could affect the progression of ovarian cancer by increasing the expression of cytotoxic T cells and inhibiting angiogenesis. In conclusion, we hope that our data will provide new insights into the development of immunotherapy and the selection of prognostic markers for patients with OC.