Single-Reaction Multiplex Reverse Transcription PCR for Detection of Zika, Chikungunya, and Dengue Viruses

• Published in: Emerging infectious diseases Vol. 22; no. 7; pp. 1295 - 1297
• Main Authors: Waggoner, Jesse J., Gresh, Lionel, Mohamed-Hadley, Alisha, Ballesteros, Gabriela, Davila, Maria Jose Vargas, Tellez, Yolanda, Sahoo, Malaya K., Balmaseda, Angel, Harris, Eva, Pinsky, Benjamin A.
• Format: Journal Article
• Language: English
• Published: United States U.S. National Center for Infectious Diseases 01.07.2016; Centers for Disease Control and Prevention
• Subjects: chikungunya virus; Chikungunya virus - isolation & purification; Dengue virus; Dengue Virus - isolation & purification; Dispatch; Expedited; Genome, Viral; Health aspects; Humans; Infection; multiplex molecular diagnostics; Multiplex Polymerase Chain Reaction - methods; Polymerase chain reaction; real-time RT-PCR; Reverse Transcriptase Polymerase Chain Reaction - methods; RNA, Viral - genetics; Sensitivity and Specificity; Single-Reaction Multiplex Reverse Transcription PCR for Detection of Zika, Chikungunya, and Dengue Viruses; Transcription (Genetics); viruses; Zika virus; Zika Virus - isolation & purification; Nicaragua; California; viruses; vector-borne infections; arboviruses; dengue virus; Zika virus; chikungunya virus; real-time RT-PCR; multiplex molecular diagnostics

Clinical manifestations of Zika virus, chikungunya virus, and dengue virus infections can be similar. To improve virus detection, streamline molecular workflow, and decrease test costs, we developed and evaluated a multiplex real-time reverse transcription PCR for these viruses.