Single-Reaction Multiplex Reverse Transcription PCR for Detection of Zika, Chikungunya, and Dengue Viruses

• Published in: Emerging infectious diseases Vol. 22; no. 7; pp. 1295 - 1297
• Main Authors: Waggoner, Jesse J., Gresh, Lionel, Mohamed-Hadley, Alisha, Ballesteros, Gabriela, Davila, Maria Jose Vargas, Tellez, Yolanda, Sahoo, Malaya K., Balmaseda, Angel, Harris, Eva, Pinsky, Benjamin A.
• Format: Journal Article
• Language: English
• Published: United States U.S. National Center for Infectious Diseases 01.07.2016; Centers for Disease Control and Prevention
• Subjects: chikungunya virus; Chikungunya virus - isolation & purification; Dengue virus; Dengue Virus - isolation & purification; Dispatch; Expedited; Genome, Viral; Health aspects; Humans; Infection; multiplex molecular diagnostics; Multiplex Polymerase Chain Reaction - methods; Polymerase chain reaction; real-time RT-PCR; Reverse Transcriptase Polymerase Chain Reaction - methods; RNA, Viral - genetics; Sensitivity and Specificity; Single-Reaction Multiplex Reverse Transcription PCR for Detection of Zika, Chikungunya, and Dengue Viruses; Transcription (Genetics); viruses; Zika virus; Zika Virus - isolation & purification; Nicaragua; California; viruses; vector-borne infections; arboviruses; dengue virus; Zika virus; chikungunya virus; real-time RT-PCR; multiplex molecular diagnostics
• ISSN: 1080-6040; 1080-6059; 1080-6059
• DOI: 10.3201/eid2207.160326

Clinical manifestations of Zika virus, chikungunya virus, and dengue virus infections can be similar. To improve virus detection, streamline molecular workflow, and decrease test costs, we developed and evaluated a multiplex real-time reverse transcription PCR for these viruses.