Quantification of the catalytic performance of C1-cellulose-specific lytic polysaccharide monooxygenases

• Published in: Applied microbiology and biotechnology Vol. 102; no. 3; pp. 1281 - 1295
• Main Authors: Frommhagen, Matthias, Westphal, Adrie H., Hilgers, Roelant, Koetsier, Martijn J., Hinz, Sandra W. A., Visser, Jaap, Gruppen, Harry, van Berkel, Willem J. H., Kabel, Mirjam A.
• Format: Journal Article
• Language: English
• Published: Berlin/Heidelberg Springer Berlin Heidelberg 01.02.2018; Springer; Springer Nature B.V
• Subjects: beta-Glucosidase - metabolism; Biodegradation; Biofuels; Biomass; Biomedical and Life Sciences; Biotechnologically Relevant Enzymes and Proteins; Biotechnology; Catalysis; Cellulose; Cellulose - metabolism; Chemical properties; Chitin - metabolism; Copper - metabolism; Ethanol; Glucosidase; Hydrogen-Ion Concentration; Life Sciences; Lignin - metabolism; Lignocellulose; Lytic polysaccharide monooxygenase; Microbial Genetics and Genomics; Microbiology; Mixed Function Oxygenases - metabolism; Observations; Oligosaccharides; Oligosaccharides - metabolism; Oxidases; Oxidation; Oxidation-Reduction; pH effects; Plant biomass; Plants - chemistry; Polysaccharides; Reducing agent; Reducing agents; Saccharides; Shells; Sordariales - enzymology; Temperature; β-Glucosidase; β-Glucosidase; Lignocellulose; Reducing agent; Lytic polysaccharide monooxygenase; Plant biomass
• ISSN: 0175-7598; 1432-0614
• DOI: 10.1007/s00253-017-8541-9

Lytic polysaccharide monooxygenases (LPMOs) have recently been shown to significantly enhance the degradation of recalcitrant polysaccharides and are of interest for the production of biochemicals and bioethanol from plant biomass. The copper-containing LPMOs utilize electrons, provided by reducing agents, to oxidatively cleave polysaccharides. Here, we report the development of a β-glucosidase-assisted method to quantify the release of C1-oxidized gluco-oligosaccharides from cellulose by two C1-oxidizing LPMOs from Myceliophthora thermophila C1. Based on this quantification method, we demonstrate that the catalytic performance of both Mt LPMOs is strongly dependent on pH and temperature. The obtained results indicate that the catalytic performance of LPMOs depends on the interaction of multiple factors, which are affected by both pH and temperature.