In-situ Neutron Reflectometry Study on Adsorption of Glucose Oxidase at Mesoporous Aluminum Oxide Film

In the present study, the adsorption of glucose oxidase (GOD) to a mesoporous aluminum oxide (MAO) film was examined with in-situ neutron reflectometry (NR) measurements. The MAO film was deposited on a cover glass slip and a Si disc, and its pore structure was characterized by X-ray reflectometry (...

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Published inAnalytical Sciences Vol. 36; no. 11; pp. 1331 - 1336
Main Authors YAMAGUCHI, Akira, KATAYAMA, Kazuya, HOLT, Stephen A.
Format Journal Article
LanguageEnglish
Published Singapore The Japan Society for Analytical Chemistry 10.11.2020
Springer Nature Singapore
Japan Science and Technology Agency
Subjects
Adsorption
Aluminum
Aluminum oxide
Aluminum Oxide - chemistry
Amine oxidase (flavin-containing)
Analytical Chemistry
Chemistry
Diameters
Glucose oxidase
Glucose Oxidase - chemistry
Mesoporous aluminum oxide
myoglobin
neutron reflectometry
Neutrons
Optical Phenomena
Oxide coatings
Porosity
Reflectometry
Surface charge
Surface Properties
adsorption
Mesoporous aluminum oxide
myoglobin
neutron reflectometry
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Summary:In the present study, the adsorption of glucose oxidase (GOD) to a mesoporous aluminum oxide (MAO) film was examined with in-situ neutron reflectometry (NR) measurements. The MAO film was deposited on a cover glass slip and a Si disc, and its pore structure was characterized by X-ray reflectometry (XRR) and NR. The Si disc with MAO film was applied for an in-situ NR experiment, and its NR profiles before/after adsorption of GOD were continuously measured with a flow cell. The results indicated that the negatively-charged GOD molecules hardly penetrate into the narrow pore channel (pore diameter = ca. 10 nm) with opposite surface charge.
Bibliography:ObjectType-Article-1
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ISSN:0910-6340
1348-2246
DOI:10.2116/analsci.20P160