Survey of molecular methods for the typing of wine yeast strains

• Published in: FEMS microbiology letters Vol. 231; no. 1; pp. 19 - 26
• Main Authors: Schuller, Dorit, Valero, Eva, Dequin, Sylvie, Casal, Margarida
• Format: Journal Article
• Language: English
• Published: Oxford, UK Elsevier B.V 09.02.2004; Blackwell Publishing Ltd; Blackwell; Wiley-Blackwell
• Subjects: Biological and medical sciences; Commercial yeast; DNA, Mitochondrial - analysis; DNA, Mitochondrial - genetics; DNA, Mitochondrial - isolation & purification; Epidemiology; Fermentation; Food Microbiology; Fundamental and applied biological sciences. Psychology; Interdelta sequence; Karyotyping; Life Sciences; Microbiology; Microbiology and Parasitology; Microsatellite; Microsatellite Repeats; Mitochondrial DNA restriction fragment length polymorphism; Molecular typing; Mycological Typing Techniques; Mycology; Pathogenicity, host-agent relations, miscellaneous strains, epidemiology; Polymorphism, Restriction Fragment Length; Virology; Wine - microbiology; Yeasts - classification; Yeasts - genetics; Yeasts - isolation & purification; Mitochondrial DNA restriction fragment length polymorphism; Microsatellite; Molecular typing; Commercial yeast; Interdelta sequence; Karyotyping; Yeast; Genotype; Mitochondrial DNA; Method; Strain; Restriction fragment length polymorphism; Microsatellite DNA; Molecular epidemiology
• ISSN: 0378-1097; 1574-6968
• DOI: 10.1016/S0378-1097(03)00928-5

A survey of the genetic polymorphisms produced by distinct methods was performed in 23 commercial winery yeast strains. Microsatellite typing, using six different loci, an optimized interdelta sequence analysis and restriction fragment length polymorphism of mitochondrial DNA generated by the enzyme HinfI had the same discriminatory power: among the 23 commercial yeast strains, 21 distinct patterns were obtained. Karyotype analysis gave 22 patterns, thereby allowing the discrimination of one of the three strains that were not distinguished by the other methods. Due to the equivalence of the results obtained in this survey, any of the methods can be applied at the industrial scale.