土壤中以抗生素为单一碳源的抗性细菌

通过单一碳源选择性培养基（sole carbon source,SCS）,从4种不同利用类型的土壤中分离得到2株以青霉素为碳源的菌株（p4,p5）以及3株以四环素为碳源的菌株（t1,t5,t9）,5种菌株均为革兰阴性菌。研究结果表明抗生素能刺激相应抗性菌株的生长。分别将p4,t5,t9菌株接种到100mg/L相应抗生素SCS培养液中,30d后培养液中抗生素质量浓度与不接种菌株的培养液相比分别下降19.5%,29.1%,24.9%。通过系统发育分析,5种菌株分别属于溶杆菌属（Lysobacter）、贪噬菌属（Variovorax）、假单胞菌属（Pseudomona）、噬几丁质菌属（Chitino...

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Published in浙江大学学报（农业与生命科学版） Vol. 42; no. 5; pp. 582 - 588
Main Author 顾超 张奇春 徐晨光
Format Journal Article
LanguageChinese
Published 浙江省亚热带土壤与植物营养重点实验室/污染环境修复与生态健康教育部重点实验室/浙江大学环境与资源学院,310058 2016
Subjects
单一碳源
抗生素
系统发育分析
抗生素
phylogenetic analysis
系统发育分析
sole carbon source
单一碳源
antibiotics
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Summary:通过单一碳源选择性培养基（sole carbon source,SCS）,从4种不同利用类型的土壤中分离得到2株以青霉素为碳源的菌株（p4,p5）以及3株以四环素为碳源的菌株（t1,t5,t9）,5种菌株均为革兰阴性菌。研究结果表明抗生素能刺激相应抗性菌株的生长。分别将p4,t5,t9菌株接种到100mg/L相应抗生素SCS培养液中,30d后培养液中抗生素质量浓度与不接种菌株的培养液相比分别下降19.5%,29.1%,24.9%。通过系统发育分析,5种菌株分别属于溶杆菌属（Lysobacter）、贪噬菌属（Variovorax）、假单胞菌属（Pseudomona）、噬几丁质菌属（Chitinophaga）和慢生根瘤菌属（Bradyrhizobium）,说明土壤中以抗生素为单一碳源分离的抗性细菌具有群落结构多样性,甚至有菌株与机会致病菌相关,应引起重视。
Bibliography:GU Chao, ZHANG Qichun, XU Chenguang （Zhejiang Provincial Key Laboratory of Subtropical Soil and Plant Nutrition/Key Laboratory of Environment Remediation and Ecological Health of the Ministry of Education/College of Environmental and Resource Sciences, Zhejiang University, Hangzhou 310058, China）
Resistance genes and strains were increased significantly with antibiotic accumulation in soil.Some resistant strains showed multiple drug resistance.Resistance genes can not only transfer between different species of bacteria,but also to humans by edible crops.Thus,the harm of resistant strains to the environment and human health is alarming.Studies showed that many microorganisms grew in an extreme environment and could degrade toxic substances,therefore,there must be some bacteria living with antibiotics as carbon sources for growth in the environment.Although many studies reported antibiotic resistant strains,little effort had been found in the literature to isolate strains with antibiotics for carbon sources. Wit
ISSN:1008-9209
DOI:10.3785/j.issn.1008-9209.2016.03.171