A protocol for Agrobacterium-mediated transformation of Japanese cedar, Sugi (Cryptomeria japonica D. Don) using embryogenic tissue explants

Sugi (Cryptomeria japonica D. Don) is the most important afforestation coniferous tree in Japan. Coniferous trees normally have a long juvenile period and require a long cultivation time for breeding. Through a traditional breeding project that began in the 1950s, first generation plus trees with ex...

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Published inPlant Biotechnology Vol. 37; no. 2; pp. 147 - 156
Main Authors Konagaya, Ken-ichi, Nanasato, Yoshihiko, Taniguchi, Toru
Format Journal Article
LanguageEnglish
Published Tokyo Japanese Society for Plant Biotechnology 25.06.2020
Japan Science and Technology Agency
Japanese Society for Plant Cell and Molecular Biology
Subjects
Afforestation
Agrobacterium
Agrobacterium tumefaciens
Breeding
conifer
Coniferous trees
Cryptomeria japonica
Cultivation
Explants
Genetic transformation
Regeneration
Review
Seedlings
Seeds
stable transformation
Transformations
Trees
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Summary:Sugi (Cryptomeria japonica D. Don) is the most important afforestation coniferous tree in Japan. Coniferous trees normally have a long juvenile period and require a long cultivation time for breeding. Through a traditional breeding project that began in the 1950s, first generation plus trees with excellent traits were selected primarily from artificial forests and used as seedlings. Recently, the second generation plus trees obtained by crossing between plus trees have been selected. In light of this situation, the improvement of Sugi by a transgenic approach is effective in terms of shortening the breeding period compared with conventional crossing-dependent approaches. There are three key points to an efficient Agrobacterium-mediated transformation system: (1) establishment of explants with high regeneration ability, (2) optimal co-cultivation conditions for explants and Agrobacterium, and (3) efficient elimination of Agrobacterium. Here we describe a protocol for Agrobacterium-mediated transformation of Sugi that meets the above criteria using embryogenic tissues as explants isolated from immature seeds obtained by crossing.
Bibliography:ObjectType-Article-1
SourceType-Scholarly Journals-1
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Present address: Tohoku Regional Office, Forest Tree Breeding Center, FFPRI, 95 Osaki, Takizawa, Iwate 020-0621, Japan
ISSN:1342-4580
1347-6114
DOI:10.5511/plantbiotechnology.20.0131a