Screening RAPD primers to assess clonal fidelity in somatic embryos of Sawara cypress (Chamaecyparis pisifera Sieb. et Zucc.) and field performance of somatic embryo-derived trees

In this study, 100 random amplified polymorphic DNA (RAPD) primers were screened to assess clonal fidelity in somatic embryos of Sawara cypress (Chamaecyparis pisifera Sieb. et Zucc.). Of the 100 primers tested, eight were selected based on their amplification products, which showed clear DNA fragme...

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Bibliographic Details
Published inPlant Biotechnology Vol. 32; no. 2; pp. 149 - 155
Main Authors Hosoi, Yoshihisa, Kuramoto, Noritsugu, Maruyama, Tsuyoshi E.
Format Journal Article
LanguageEnglish
Published Tokyo Japanese Society for Plant Cell and Molecular Biology 01.01.2015
Japan Science and Technology Agency
Subjects
Chamaecyparis pisifera
Cupressus
field performance
genetic stability
RAPD primers
Sawara cypress
somatic embryogenesis
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Summary:In this study, 100 random amplified polymorphic DNA (RAPD) primers were screened to assess clonal fidelity in somatic embryos of Sawara cypress (Chamaecyparis pisifera Sieb. et Zucc.). Of the 100 primers tested, eight were selected based on their amplification products, which showed clear DNA fragmentation, generating 8.75 bands per primer in average. The amplification products showed no genetic variation among the tested somatic embryos. In addition, the performance of somatic embryo-derived trees was monitored in the field, and their mean height growth was measured until the age of 10 years. Although no phenotypic differences were observed, the somatic embryo-derived trees showed lower growth compared with seedlings.
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content type line 23
ISSN:1342-4580
1347-6114
DOI:10.5511/plantbiotechnology.15.0408a