Simultaneous Detection of Six Different Types of Pesticides by an Immunosensor Based on Surface Plasmon Resonance

• Published in: Analytical Sciences Vol. 36; no. 3; pp. 335 - 340
• Main Authors: MIYAKE, Shiro, HIRAKAWA, Yuki, YAMASAKI, Tomomi, WATANABE, Eiki, HARADA, Ayako, IWASA, Seiji, NARITA, Hiroshi
• Format: Journal Article
• Language: English
• Published: Singapore The Japan Society for Analytical Chemistry 10.03.2020; Springer Nature Singapore; Japan Science and Technology Agency
• Subjects: Analytical Chemistry; Azoxystrobin; Biosensing Techniques; Chemistry; Chlorfenapyr; Correlation coefficient; Correlation coefficients; Environmental Monitoring - methods; Environmental Pollutants - analysis; fungicide; High performance liquid chromatography; Immunosensor; Immunosensors; insecticide; Liquid chromatography; Monoclonal antibodies; monoclonal antibody; pesticide; Pesticide residues; Pesticide Residues - analysis; Pesticides; Pesticides - analysis; Resonance; Surface plasmon resonance; Surface Plasmon Resonance - methods; Tomatoes; Vegetables; insecticide; fungicide; monoclonal antibody; surface plasmon resonance; pesticide; Immunosensor
• ISSN: 0910-6340; 1348-2246
• DOI: 10.2116/analsci.19P333

Six pesticides, azoxystrobin, boscalid, chlorfenapyr, imazalil, isoxathion, and nitenpyram, were simultaneously detected by using a surface plasmon resonance (SPR) immunosensor. The working ranges were 3.5 – 19 ng/mL for azoxystrobin, 4.5 – 50 ng/mL for boscalid, 2.5 – 25 ng/mL for chlorfenapyr, 5.5 – 50 ng/mL for imazalil, 3.5 – 50 ng/mL for isoxathion, and 8.5 – 110 ng/mL for nitenpyram. They showed adequate recovery results in tomato samples: 104 – 116% for azoxystrobin, 94 – 101% for boscalid, 90 – 112% for chlorfenapyr, 96 – 106% for imazalil, 107 – 119% for isoxathion, and 104 – 109% for nitenpyram. The correlation coefficient with liquid chromatography (HPLC or LC-MS/MS) using vegetable samples also agreed well: 0.91 – 0.99 as R2 without strong bias, except for nitenpyram for which the SPR immunosensor sensitivity was too low. The SPR immunosensor will have high applicability for pesticide residue analyses in vegetable samples.