DNA polymorphism of srRNA gene among Eimeria tenella strains isolated in Japan

• Published in: Journal of Veterinary Medical Science Vol. 61; no. 12; pp. 1331 - 1333
• Main Authors: Tsuji, N. (National Inst. of Animal Health, Tsukuba, Ibaraki (Japan)), Ohta, M, Kawazu, S, Kamio, T, Isobe, T, Shimura, K, Fujisaki, K
• Format: Journal Article
• Language: English
• Published: Japan JAPANESE SOCIETY OF VETERINARY SCIENCE 01.12.1999; Japan Science and Technology Agency
• Subjects: ADN; Animals; ARN RIBOSOMAL; ARN RIBOSOMIAL; BIOLOGICAL DIFFERENCES; CHICKENS; Coccidiosis - parasitology; Coccidiosis - veterinary; DIFERENCIAS BIOLOGICAS; DIFFERENCE BIOLOGIQUE; DNA; DNA polymorphism; DNA Primers - chemistry; DNA, Protozoan - chemistry; DNA, Protozoan - genetics; EIMERIA TENELLA; Eimeria tenella - chemistry; Eimeria tenella - classification; Eimeria tenella - genetics; Electrophoresis, Agar Gel - veterinary; GENE; GENES; GENETIC POLYMORPHISM; Genetic Variation - genetics; JAPAN; JAPON; POLIMORFISMO GENETICO; POLLO; Polymerase Chain Reaction - veterinary; Polymorphism, Genetic - genetics; POLYMORPHISME GENETIQUE; POULET; Poultry Diseases - parasitology; Random Amplified Polymorphic DNA Technique - veterinary; RAPD; RIBOSOMAL RNA; RNA, Protozoan - chemistry; RNA, Protozoan - genetics; RNA, Ribosomal - chemistry; RNA, Ribosomal - genetics; Specific Pathogen-Free Organisms; Japan
• ISSN: 0916-7250; 1347-7439
• DOI: 10.1292/jvms.61.1331

DNA polymorphism in twelve starains of Eimeria tenella isolated from various places in Japan was examined using 1.1 kb small subunits ribosomal RNA amplified by PCR. Genetic variation was evaluated by random amplification of polymorphic DNA (RAPD) analysis. DNA fingerprint patterns were grouped into two, indicating that at least two DNA polymorphisms exist in Japanese E. tenella strains