Decolourization of azo dyes and a dye industry effluent by a white rot fungus Thelephora sp

• Published in: Bioresource technology Vol. 88; no. 2; pp. 115 - 119
• Main Authors: Selvam, K., Swaminathan, K., Chae, Keon-Sang
• Format: Journal Article
• Language: English
• Published: Oxford Elsevier Ltd 01.06.2003; Elsevier Science
• Subjects: Azo Compounds - metabolism; Azo Dyes; Basidiomycota - physiology; Biodegradation, Environmental; Biological and medical sciences; Chemical Industry; Coloring Agents - metabolism; decolorization; Decolourization; Dye industry effluent; effluents; Fundamental and applied biological sciences. Psychology; Industrial Waste; industry; Laccase; lignin peroxidase; Lignin peroxidase (LiP); Manganese dependent peroxidase (MnP); manganese peroxidase; peroxidase; Thelephora; Thelephora sp; Waste Disposal, Fluid; Water Purification - methods; white-rot fungi; Manganese dependent peroxidase (MnP); Decolourization; Thelephora sp; Lignin peroxidase (LiP); Azo dyes; Dye industry effluent; Laccase; Enzyme; Manganese peroxidase; Decolorizing process; Fungi; Industry; Peroxidases; Azo dye; Rot; Oxidoreductases; Effluent; Thallophyta; Diarylpropane peroxidase
• ISSN: 0960-8524; 1873-2976
• DOI: 10.1016/S0960-8524(02)00280-8

A white rot fungus Thelephora sp. was used for decolourization of azo dyes such as orange G (50 μM), congo red (50 μM), and amido black 10B (25 μM). Decolourization using the fungus was 33.3%, 97.1% and 98.8% for orange G, congo red and amido black 10B, respectively. An enzymatic dye decolourization study showed that a maximum of 19% orange G was removed by laccase at 15 U/ml whereas lignin peroxidase (LiP) and manganese dependent peroxidase (MnP) at the same concentration decolourized 13.5% and 10.8%, orange G, respectively. A maximum decolourization of 12.0% and 15.0% for congo red and amido black 10B, respectively, was recorded by laccase. A dye industry effluent was treated by the fungus in batch and continuous modes. A maximum decolourization of 61% was achieved on the third day in the batch mode and a maximum decolourization of 50% was obtained by the seventh day in the continuous mode. These results suggest that the batch mode of treatment using Thelephora sp. may be more effective than the continuous mode for colour removal from dye industry effluents.