Isolation of aino virus from an aborted bovine fetus

A male fetus of gestation day 187 was aborted from a Holstein-Friesian cow in an epizootic of the Aino virus (AINOV) in September 1995. Neutralizing antibody titers against AINOV were 1:128, 1:16 and 1:64 in the dam serum, fetal ascites and cerebrospinal fluid, respectively. A 10% brain suspension o...

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Published inJournal of Veterinary Medical Science Vol. 60; no. 10; pp. 1139 - 1140
Main Authors Uchinuno, Y. (Fukuoka-ken. Government Office (Japan)), Noda, Y, Ishibashi, K, Nagasue, S, Shirakawa, H, Nagano, M, Ohe, R
Format Journal Article
LanguageEnglish
Published Japan JAPANESE SOCIETY OF VETERINARY SCIENCE 1998
Japan Science and Technology Agency
Subjects
aborted bovine fetus
ABORTION
Abortion, Veterinary - virology
ABORTO
Aino virus
ANALISIS MICROBIOLOGICO
ANALYSE MICROBIOLOGIQUE
ANIMAL VIRUSES
Animals
Antibodies, Viral - analysis
AVORTEMENT
BOVIN
Brain - embryology
Brain - virology
Bunyaviridae Infections - epidemiology
Bunyaviridae Infections - veterinary
Bunyaviridae Infections - virology
CATTLE
Cattle Diseases - epidemiology
Cattle Diseases - virology
Cytopathogenic Effect, Viral
Female
Fetal Death - veterinary
Fetal Death - virology
FETO
Fetus - virology
FOETUS
GANADO BOVINO
Male
MICROBIOLOGICAL ANALYSIS
Pregnancy
Sentinel Surveillance - veterinary
Seroepidemiologic Studies
Simbu virus - classification
Simbu virus - immunology
Simbu virus - isolation & purification
VIRUS DE LOS ANIMALES
VIRUS DES ANIMAUX
virus isolation
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Summary:A male fetus of gestation day 187 was aborted from a Holstein-Friesian cow in an epizootic of the Aino virus (AINOV) in September 1995. Neutralizing antibody titers against AINOV were 1:128, 1:16 and 1:64 in the dam serum, fetal ascites and cerebrospinal fluid, respectively. A 10% brain suspension of the aborted fetus was prepared immediately after autopsy, rinsed three times and sonicated before centrifugation. The supernatant was then inoculated into HmLu-1 cell cultures. A cytopathic effect was noted on post-inoculation day 7. The isolated virus was identified as the AINOV based on the physicochemical properties and cross neutralization test. This is the first report on the isolation of AINOV from an aborted bovine fetus.
Bibliography:L73
1999001106
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ISSN:0916-7250
1347-7439
DOI:10.1292/jvms.60.1139