Reproducible insulin secretion from isolated rat pancreas preparations using an organ bath

• Published in: Experimental Animals Vol. 67; no. 1; pp. 15 - 22
• Main Authors: Morita, Asuka, Ouchi, Motoshi, Terada, Misao, Kon, Hiroe, Kishimoto, Satoko, Satoh, Keitaro, Otani, Naoyuki, Hayashi, Keitaro, Fujita, Tomoe, Inoue, Ken-ichi, Anzai, Naohiko
• Format: Journal Article
• Language: English
• Published: Japan Japanese Association for Laboratory Animal Science 01.01.2018; Japan Science and Technology Agency
• Subjects: amylase; Amylases - metabolism; Animals; Cannulation; Diabetes mellitus; Glucagon-Like Peptide 1 - pharmacology; glucagon-like peptide-1; Glucose; Hypoglycemic agents; In Vitro Techniques; Insulin; Insulin - metabolism; Insulin Secretion; Insulin-Secreting Cells - metabolism; Male; organ bath; Organ Culture Techniques - methods; Original; Pancreas; Pancreas - cytology; Pharmacology; Potassium; Quantitative analysis; rat pancreas; Rats, Wistar; Reproducibility of Results; Secretion; rat pancreas; insulin; amylase; glucagon-like peptide-1; organ bath
• ISSN: 1341-1357; 1881-7122
• DOI: 10.1538/expanim.17-0059

Diabetes mellitus is a lifestyle-related disease that is characterized by inappropriate or diminished insulin secretion. Ex vivo pharmacological studies of hypoglycemic agents are often conducted using perfused pancreatic preparations. Pancreas preparations for organ bath experiments do not require cannulation and are therefore less complex than isolated perfused pancreas preparations. However, previous research has generated almost no data on insulin secretion from pancreas preparations using organ bath preparations. The purpose of this study was to investigate the applicability of isolated rat pancreas preparations using the organ bath technique in the quantitative analysis of insulin secretion from β-cells. We found that insulin secretion significantly declined during incubation in the organ bath, whereas it was maintained in the presence of 1 µM GLP-1. Conversely, amylase secretion exhibited a modest increase during incubation and was not altered in the presence of GLP-1. These results demonstrate that the pancreatic organ bath preparation is a sensitive and reproducible method for the ex vivo assessment of the pharmacological properties of hypoglycemic agents.