Upregulated expression of serum exosomal miR-375 and miR-1307 enhance the diagnostic power of CA125 for ovarian cancer

• Published in: Journal of ovarian research Vol. 12; no. 1; p. 6
• Main Authors: Su, Ying Ying, Sun, Li, Guo, Zhi Rui, Li, Jin Chang, Bai, Ting Ting, Cai, Xiao Xiao, Li, Wen Han, Zhu, Ye Fei
• Format: Journal Article
• Language: English
• Published: England BioMed Central Ltd 22.01.2019; BioMed Central; BMC
• Subjects: Abdomen; Adult; Aged; Aged, 80 and over; Biological markers; Biomarker; Biomarkers; Biomarkers, Tumor - blood; Biomarkers, Tumor - genetics; Breast cancer; Cancer metastasis; Cancer staging; Cancer therapies; Chemotherapy; Development and progression; Diagnosis; DNA methylation; Exosomes; Exosomes - genetics; Female; Gene expression; Genetic aspects; Gynecology; Humans; Lymph nodes; Lymphatic Metastasis - genetics; Mediation; Medical prognosis; Metastases; Metastasis; MicroRNA; MicroRNAs; MicroRNAs - blood; Middle Aged; miRNA; Mortality; Neoplasm Staging; Oncology; Ovarian cancer; Ovarian Neoplasms - blood; Ovarian Neoplasms - diagnosis; Ovarian Neoplasms - genetics; Ovarian Neoplasms - pathology; Patient outcomes; Serum exosome; Tumors; Up-Regulation; Young Adult; microRNA; Biomarker; Serum exosome; Ovarian cancer
• ISSN: 1757-2215; 1757-2215
• DOI: 10.1186/s13048-018-0477-x

Ovarian cancer (OC) is associated with high mortality in gynecological oncology; this is mainly due to the low diagnosis rate. Exosomal miRNA has demonstrated potential as a tumor biomarker. We aimed to explore the diagnostic potential of serum exosomal miR-1307 and miR-375 for OC. The first six candidate miRNAs were selected from the previous literature. The relative quantification of qRT-PCR was used to screen for the stability of exosomal miRNAs, followed by validation of the cohort. ROC analysis was employed to analyze the specificity and sensitivity of exosomal miRNA. MiR-1307 and miR-375 were confirmed stably existing in serum exosomes of OC. Moreover, miR-1307 and miR-375 were both significantly up-regulated in serum exosomes of OC compared to ovarian benign and healthy groups. The overexpressed miRNAs showed independent diagnostic power and enhanced the diagnostic accuracy of traditional biomarkers when combined with CA-125 and HE4. MiR-1307 was associated with tumor staging, and miR-375 was associated with lymph node metastasis of OC. Our results suggest that serum exosomal miR-1307 and miR-375 could serve as potential tumor biomarkers to improve diagnostic efficiency for OC.