Isolation of STLV–I from Orangutan, a Great Ape Species in Southeast Asia, and Its Relation to Other HTLV–Is/STLV–Is

• Published in: Cancer science Vol. 88; no. 1; pp. 1 - 4
• Main Authors: Ibuki, Kentaro, Ido, Eiji, Setiyaningsih, Surachmi, Yamashita, Masahiro, Agus, Lelana R. P., Takehisa, Jun, Miura, Tomoyuki, Dondin, Sajuthi, Hayami, Masanori
• Format: Journal Article
• Language: English
• Published: Oxford, UK Blackwell Publishing Ltd 01.01.1997; Japanese Cancer Association; John Wiley & Sons, Inc
• Subjects: Agglutination; AIDS/HIV; Animals; Base Sequence; Biological and medical sciences; Divergence; Fundamental and applied biological sciences. Psychology; Human T-lymphotropic virus 1 - classification; Human T-lymphotropic virus 1 - genetics; Immunofluorescence; Indonesia; Leukocytes (mononuclear); Long terminal repeat; LTR; Microbiology; Molecular Sequence Data; Monkeys & apes; Orangutan; Peripheral blood mononuclear cells; Phylogenetic analysis; Phylogeny; Polymerase chain reaction; Pongo pygmaeus; Pongo pygmaeus - virology; Replicative cycle, interference, host-virus relations, pathogenicity, miscellaneous strains; simian T-lymphotropic virus; Simian T-lymphotropic virus 1 - classification; Simian T-lymphotropic virus 1 - genetics; Simian T-lymphotropic virus 1 - isolation & purification; STLV–I/HTLV; Virology; Western blotting; Asia; Indonesia; Virus; Vertebrata; STLV1 virus; Mammalia; Animal; Monkey; Primates; Retroviridae; Simioidea; Phylogeny; HTLV-I virus; Pongo pygmaeus
• ISSN: 0910-5050; 1347-9032; 1349-7006; 1876-4673
• DOI: 10.1111/j.1349-7006.1997.tb00293.x

To study the evolutionary origin of human T–lymphotropic virus type I/simian T–lymphotropic virus type I (HTLV–I/STLV–I), we isolated and characterized STLV–I from orangutans (Pongo pygmaeus). Plasma samples from 3 out of 41 animals examined were reactive by particle agglutination and immunofluorescence, and one of these three was confirmed to be anti–HTLV–I antibody–positive by western blotting (WB). Cultured peripheral blood mononuclear cells from the WB–positive orangutan were reactive to anti–STLV–I–positive rhesus monkey plasma. The proviral long terminal repeat region was amplified by polymerase chain reaction and sequenced. A phylogenetic analysis indicated that orangutan STLV–I is related to the Melanesian group of HTLV–Is and other Asian STLV–Is, but the degree of divergence is considerable.