Isolation of a New Virus, HBLV, in Patients with Lymphoproliferative Disorders

• Published in: Science (American Association for the Advancement of Science) Vol. 234; no. 4776; pp. 596 - 601
• Main Authors: Salahuddin, S. Zaki, Ablashi, Dharam V., Markham, Phillip D., Josephs, Steven F., Sturzenegger, Susi, Kaplan, Mark, Halligan, Gregory, Biberfeld, Peter, Wong-Staal, Flossie, Kramarsky, Bernhard, Gallo, Robert C.
• Format: Journal Article
• Language: English
• Published: Washington, DC The American Association for the Advancement of Science 31.10.1986; American Association for the Advancement of Science
• Subjects: AIDS (Disease); AIDS research; AIDS/HIV; Animals; B lymphocytes; B-Lymphocytes - microbiology; Biological and medical sciences; Blood cells; Cell culture techniques; Cell Line; Cell lines; Deltaretrovirus Infections - microbiology; Epithelial cells; Epstein Barr virus infections; Fluorescent Antibody Technique; Fundamental and applied biological sciences. Psychology; Haplorhini; Herpes viruses; Herpesviridae; Herpesviridae - isolation & purification; Herpesviridae Infections - microbiology; Herpesvirus; Herpesviruses; Human herpesvirus 6; Humans; Innovations; Lymphoproliferative Disorders - microbiology; Medical research; Microbiology; Microscopy, Electron; Primates; Systematics; T lymphocytes; T-Lymphocytes - microbiology; Viral research; Virology; Virus research; Viruses; Tropism; Cell culture; Antigenic relationship; Antibody; Leukocyte; Herpesviridae; HBLV virus; AIDS; Hemopathy; B-Lymphocyte; Immunological method; Infection; Virus; Transmission electron microscopy; Viral disease; Lymphoproliferative syndrome; Morphology; Infectivity; Classification; Cytopathology; Isolation; Serum; Immunofluorescence
• ISSN: 0036-8075; 1095-9203
• DOI: 10.1126/science.2876520

A novel human B-lymphotropic virus (HBLV) was isolated from the peripheral blood leukocytes of six individuals: two HTLV-III seropositive patients from the United States (one with AIDS-related lymphoma and one with dermatopathic lymphadenopathy), three HTLV-III seronegative patients from the United States (one with angioimmunoblastic lymphadenopathy, one with cutaneous T-cell lymphoma, and one with immunoblastic lymphoma), and one HTLV-III seronegative patient with acute lymphocytic leukemia from Jamaica. All six isolates were closely related by antigenic analysis, and sera from all six virus-positive patients reacted immunologically with each virus isolate. In contrast, only four sera from 220 randomly selected healthy donors and none from 12 AIDS patients without associated lymphoma were seropositive. The virus selectively infected freshly isolated human B cells and converted them into large, refractile mono- or binucleated cells with nuclear and cytoplasmic inclusion bodies. HBLV is morphologically similar to viruses of the herpesvirus family but is readily distinguishable from the known human and nonhuman primate herpesviruses by host range, in vitro biological effects, and antigenic features.