role for pectin de‐methylesterification in a developmentally regulated growth acceleration in dark‐grown Arabidopsis hypocotyls

• Published in: The New phytologist Vol. 188; no. 3; pp. 726 - 739
• Main Authors: Pelletier, Sandra, Van Orden, Jürgen, Wolf, Sebastian, Vissenberg, Kris, Delacourt, Julien, Ndong, Yves Assoumou, Pelloux, Jérôme, Bischoff, Volker, Urbain, Aurélie, Mouille, Grégory, Lemonnier, Gaëtan, Renou, Jean‐Pierre, Höfte, Herman
• Format: Journal Article
• Language: English
• Published: Oxford, UK Blackwell Publishing Ltd 01.11.2010; John Wiley & Sons; Wiley Subscription Services, Inc; Wiley
• Subjects: Acceleration; Analysis; Arabidopsis; Arabidopsis - cytology; Arabidopsis - growth & development; Arabidopsis - metabolism; Arabidopsis thaliana; Carboxylic Ester Hydrolases - antagonists & inhibitors; cell elongation; Cell growth; cell wall; Cell Wall - metabolism; Cell walls; Cellulose; Cellulose - biosynthesis; Darkness; Dry cleaners; Elongation; Esterification; Fourier analysis; Fourier transform infrared (FT‐IR) microspectroscopy; Fourier transforms; Gene Expression; Gene Expression Profiling; Gene Expression Regulation, Developmental; Genes; Growth; hypocotyl; Hypocotyl - cytology; Hypocotyl - growth & development; Hypocotyl - metabolism; Hypocotyls; Infrared analysis; Inhibitors; Life Sciences; Metabolism; Oligonucleotide Array Sequence Analysis; Pectin; pectin de‐methylesterification; Pectinesterase; Pectins - metabolism; Phytopathology and phytopharmacy; Plant cells; Plant physiology; Plants; Polymerase chain reaction; Seedlings; Spectroscopy, Fourier Transform Infrared; Synthesis; transcriptome; Transcriptomes; Up regulation; Vegetal Biology; cell elongation; séquence; transcriptome; réponse; racines; cell wall; fourier transform infrared (ft-ir) microspectroscopy; hypocotyl; pectin de-methylesterification; résistance
• ISSN: 0028-646X; 1469-8137
• DOI: 10.1111/j.1469-8137.2010.03409.x

• We focused on a developmentally regulated growth acceleration in the dark‐grown Arabidopsis hypocotyl to study the role of changes in cell wall metabolism in the control of cell elongation. • To this end, precise transcriptome analysis on dissected dark‐grown hypocotyls, Fourier transform infrared (FT‐IR) microspectroscopy and kinematic analysis were used. • Using a cellulose synthesis inhibitor, we showed that the growth acceleration marks a developmental transition during which growth becomes uncoupled from cellulose synthesis. We next investigated the cellular changes that take place during this transition. FT‐IR microspectroscopy revealed significant changes in cell wall composition during, but not after, the growth acceleration. Transcriptome analysis suggested a role for cell wall remodeling, in particular pectin modification, in this growth acceleration. This was confirmed by the overexpression of a pectin methylesterase inhibitor, which caused a delay in the growth acceleration. • This study shows that the acceleration of cell elongation marks a developmental transition in dark‐grown hypocotyl cells and supports a role for pectin de‐methylesterification in the timing of this event.