Electric field stimulation through a biodegradable polypyrrole-co-polycaprolactone substrate enhances neural cell growth

• Published in: Journal of biomedical materials research. Part A Vol. 102; no. 8; pp. 2554 - 2564
• Main Authors: Nguyen, Hieu T., Sapp, Shawn, Wei, Claudia, Chow, Jacqueline K., Nguyen, Alvin, Coursen, Jeff, Luebben, Silvia, Chang, Emily, Ross, Robert, Schmidt, Christine E.
• Format: Journal Article
• Language: English
• Published: Hoboken, NJ Blackwell Publishing Ltd 01.08.2014; Wiley-Blackwell; Wiley Subscription Services, Inc
• Subjects: Animals; Axons; Axons - drug effects; Axons - metabolism; Biocompatible Materials - chemical synthesis; Biocompatible Materials - chemistry; Biocompatible Materials - pharmacology; Biodegradability; Biological and medical sciences; Caproates - chemical synthesis; Caproates - chemistry; Caproates - pharmacology; Cell Adhesion - drug effects; Cell Count; Cell Death - drug effects; Cell Proliferation - drug effects; cell stimulation; Cell Survival - drug effects; degradable polypyrrole; Devices; Electric Conductivity; electric field; Electric fields; Electric Stimulation; Electrically conductive; Electrodes; Exposure; Ganglia, Spinal - cytology; Ganglia, Spinal - drug effects; Hydrogen-Ion Concentration; Lactones - chemical synthesis; Lactones - chemistry; Lactones - pharmacokinetics; Medical sciences; Molecular Weight; nerve conduit; nerve regeneration; Nerves; Neurons - cytology; Neurons - drug effects; Neurons - metabolism; Neurosurgery; PC12 Cells; Polymers - chemical synthesis; Polymers - chemistry; Polymers - pharmacokinetics; Polymers - pharmacology; Pyrroles - chemical synthesis; Pyrroles - chemistry; Pyrroles - pharmacology; Radiotherapy. Instrumental treatment. Physiotherapy. Reeducation. Rehabilitation, orthophony, crenotherapy. Diet therapy and various other treatments (general aspects); Rats; Rats, Sprague-Dawley; Spectroscopy, Fourier Transform Infrared; Stimulation; Surgery (general aspects). Transplantations, organ and tissue grafts. Graft diseases; Technology. Biomaterials. Equipments; Cell proliferation; nerve conduit; Aromatic polymer; Instrumentation therapy; Lactone polymer; degradable polypyrrole; Instrumental stimulation; Electric field; Aliphatic polymer; cell stimulation; Biomaterial; Polycaprolactone; Pyrrole polymer; Nerve regeneration; Biomedical engineering; electric field; nerve regeneration
• ISSN: 1549-3296; 1552-4965
• DOI: 10.1002/jbm.a.34925

Nerve guidance conduits (NGCs) are FDA‐approved devices used to bridge gaps across severed nerve cables and help direct axons sprouting from the proximal end toward the distal stump. In this article, we present the development of a novel electrically conductive, biodegradable NGC made from a polypyrrole‐block‐polycaprolactone (PPy‐PCL) copolymer material laminated with poly(lactic‐co‐glycolic acid) (PLGA). The PPy‐PCL has a bulk conductivity ranging 10–20 S/cm and loses 40 wt % after 7 months under physiologic conditions. Dorsal root ganglia (DRG) grown on flat PPy‐PCL/PLGA material exposed to direct current electric fields (EF) of 100 mV/cm for 2 h increased axon growth by 13% (± 2%) toward either electrode of a 2‐electrode setup, compared with control grown on identical substrates without EF exposure. Alternating current increased axon growth by 21% (±3%) without an observable directional preference, compared with the same control group. The results from this study demonstrate PLGA‐coated PPy‐PCL is a unique biodegradable material that can deliver substrate EF stimulation to improve axon growth for peripheral nerve repair. © 2013 Wiley Periodicals, Inc. J Biomed Mater Res Part A: 102A: 2554–2564, 2014.