Error-correcting barcoded primers for pyrosequencing hundreds of samples in multiplex

We constructed error-correcting DNA barcodes that allow one run of a massively parallel pyrosequencer to process up to 1,544 samples simultaneously. Using these barcodes we processed bacterial 16S rRNA gene sequences representing microbial communities in 286 environmental samples, corrected 92% of s...

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Bibliographic Details
Published inNature methods Vol. 5; no. 3; pp. 235 - 237
Main Authors Knight, Rob, Hamady, Micah, Walker, Jeffrey J, Harris, J Kirk, Gold, Nicholas J
Format Journal Article
LanguageEnglish
Published New York Nature Publishing Group US 01.03.2008
Nature Publishing Group
Subjects
Bioinformatics
Biological Microscopy
Biological Techniques
Biomedical and Life Sciences
Biomedical Engineering/Biotechnology
Biomedical research
brief-communication
Deoxyribonucleic acid
DNA
DNA Primers - chemistry
DNA sequencing
Genetic Code
Genetics
Life Sciences
Methods
Microbial activity
Nucleotide sequencing
Physiological aspects
Polymerase chain reaction
Primers (Molecular genetics)
Proteomics
Research methodology
RNA, Bacterial - chemistry
RNA, Ribosomal, 16S - chemistry
Sequence Analysis, DNA - methods
United States
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Summary:We constructed error-correcting DNA barcodes that allow one run of a massively parallel pyrosequencer to process up to 1,544 samples simultaneously. Using these barcodes we processed bacterial 16S rRNA gene sequences representing microbial communities in 286 environmental samples, corrected 92% of sample assignment errors, and thus characterized nearly as many 16S rRNA genes as have been sequenced to date by Sanger sequencing.
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SourceType-Scholarly Journals-1
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ISSN:1548-7091
1548-7105
DOI:10.1038/nmeth.1184