Changes induced by the Pepper mild mottle tobamovirus on the chloroplast proteome of Nicotiana benthamiana

• Published in: Photosynthesis research Vol. 103; no. 1; pp. 31 - 45
• Main Authors: Pineda, M, Sajnani, C, Barón, M
• Format: Journal Article
• Language: English
• Published: Dordrecht Dordrecht : Springer Netherlands 2010; Springer Netherlands; Springer; Springer Nature B.V
• Subjects: Analysis; Biochemistry; Biomedical and Life Sciences; Biopolymers; Calvin cycle; Cellular biology; Chloroplasts; Chloroplasts - metabolism; Electron transport; Electron Transport - physiology; Ethylenediaminetetraacetic acid; Gel electrophoresis; Health aspects; Hydrogen-Ion Concentration; Infection; Isoforms; Life Sciences; Mass spectrometry; Mass spectroscopy; Medical research; Medicine, Experimental; Nicotiana - metabolism; Nicotiana - virology; Nicotiana benthamiana; Photosynthesis; Plant biology; Plant Genetics and Genomics; Plant Physiology; Plant Proteins - metabolism; Plant Sciences; Proteins; Proteome; Proteomes; Proteomics; Regular Paper; Tobamovirus; Tobamovirus - physiology; Toy industry; Virus diseases; Biotic stress; Chloroplast proteome; Multiprotein families; Two-dimensional gel electrophoresis

We have analyzed the chloroplast proteome of Nicotiana benthamiana using two-dimensional gel electrophoresis and mass spectrometry followed by a database search. In order to improve the resolution of the two-dimensional electrophoresis gels, we have made separate maps for the low and the high pH ran...