Changes induced by the Pepper mild mottle tobamovirus on the chloroplast proteome of Nicotiana benthamiana

• Published in: Photosynthesis research Vol. 103; no. 1; pp. 31 - 45
• Main Authors: Pineda, M, Sajnani, C, Barón, M
• Format: Journal Article
• Language: English
• Published: Dordrecht Dordrecht : Springer Netherlands 2010; Springer Netherlands; Springer; Springer Nature B.V
• Subjects: Analysis; Biochemistry; Biomedical and Life Sciences; Biopolymers; Calvin cycle; Cellular biology; Chloroplasts; Chloroplasts - metabolism; Electron transport; Electron Transport - physiology; Ethylenediaminetetraacetic acid; Gel electrophoresis; Health aspects; Hydrogen-Ion Concentration; Infection; Isoforms; Life Sciences; Mass spectrometry; Mass spectroscopy; Medical research; Medicine, Experimental; Nicotiana - metabolism; Nicotiana - virology; Nicotiana benthamiana; Photosynthesis; Plant biology; Plant Genetics and Genomics; Plant Physiology; Plant Proteins - metabolism; Plant Sciences; Proteins; Proteome; Proteomes; Proteomics; Regular Paper; Tobamovirus; Tobamovirus - physiology; Toy industry; Virus diseases; Biotic stress; Chloroplast proteome; Multiprotein families; Two-dimensional gel electrophoresis
• ISSN: 0166-8595; 1573-5079
• DOI: 10.1007/s11120-009-9499-y

We have analyzed the chloroplast proteome of Nicotiana benthamiana using two-dimensional gel electrophoresis and mass spectrometry followed by a database search. In order to improve the resolution of the two-dimensional electrophoresis gels, we have made separate maps for the low and the high pH range. At least 200 spots were detected. We identified 72 polypeptides, some being isoforms of different multiprotein families. In addition, changes in this chloroplast proteome induced by the infection with the Spanish strain of the Pepper mild mottle virus were investigated. Viral infection induced the down-regulation of several chloroplastidic proteins involved in both the photosynthetic electron-transport chain and the Benson-Calvin cycle.