Double Staining of Ginsenosides by Western Blotting Using Anti-ginsenoside Rb1 and Rg1 Monoclonal Antibodies

• Published in: Biological & pharmaceutical bulletin Vol. 24; no. 10; pp. 1157 - 1160
• Main Authors: FUKUDA, Noriko, TANAKA, Hiroyuki, SHOYAMA, Yukihiro
• Format: Journal Article
• Language: English
• Published: Tokyo The Pharmaceutical Society of Japan 01.10.2001; Maruzen; Japan Science and Technology Agency
• Subjects: Analysis; Antibodies, Monoclonal - immunology; Biological and medical sciences; Blotting, Western; General pharmacology; ginseng; ginsenoside; Ginsenosides; identification; Medical sciences; monoclonal antibody(MAb); Panax - chemistry; Pharmacology. Drug treatments; Plant Extracts - chemistry; Plant Roots - chemistry; Saponins - chemistry; Saponins - immunology; Western blotting; Diol; Purification; Immunoaffinity chromatography; Monoclonal antibody; Panax ginseng; Separation method; Dicotyledones; Angiospermae; Immunoblotting assay; Spermatophyta; Ginseng; Triol; Araliaceae
• ISSN: 0918-6158; 1347-5215
• DOI: 10.1248/bpb.24.1157

Ginsenosides separated by silica gel TLC blotted to a polyvinylidene difluoride (PVDF) membrane was treated with a NaIO4 solution followed by bovine serum albumin (BSA), resulting in a ginsenoside-BSA conjugate on a PVDF membrane. The blotted bands were stained with anti-ginsenoside Rg1 and Rb1 monoclonal antibodies (MAbs). The newly established double staining with the Western blotting methods was applied for the determination of ginsenosides possessing protopanaxadiol or protopanaxatriol and the number of sugar depending on the stained color and their Rf values in the Panax plants.