Recovering the Genetic Identity of an Extinct-in-the-Wild Species: The Puzzling Case of the Alagoas Curassow

• Published in: PloS one Vol. 12; no. 1; p. e0169636
• Main Authors: Costa, Mariellen C, Oliveira, Jr, Paulo R R, Davanço, Paulo V, Camargo, Crisley de, Laganaro, Natasha M, Azeredo, Roberto A, Simpson, James, Silveira, Luis F, Francisco, Mercival R
• Format: Journal Article
• Language: English
• Published: United States Public Library of Science 05.01.2017; Public Library of Science (PLoS)
• Subjects: Alagoas curassow; Animals; Bayes Theorem; Bayesian analysis; Biology and life sciences; Clustering; Conservation; Conservation biology; Coturnix; Cracidae; Deoxyribonucleic acid; Diagnostic systems; DNA; DNA, Mitochondrial - genetics; Galliformes - classification; Galliformes - genetics; Genetic aspects; Genetic testing; Genetic Variation - genetics; Genomes; Genotype; Haplotypes; Haplotypes - genetics; Hybridization; Hybrids; Males; Microsatellite Repeats - genetics; Microsatellites; Mitochondrial DNA; Pedigree; Population; Protection and preservation; Research and Analysis Methods; Sexual behavior; Species; Taxa; Wildlife conservation
• ISSN: 1932-6203; 1932-6203
• DOI: 10.1371/journal.pone.0169636

The conservation of many endangered taxa relies on hybrid identification, and when hybrids become morphologically indistinguishable from the parental species, the use of molecular markers can assign individual admixture levels. Here, we present the puzzling case of the extinct in the wild Alagoas Curassow (Pauxi mitu), whose captive population descends from only three individuals. Hybridization with the Razor-billed Curassow (P. tuberosa) began more than eight generations ago, and admixture uncertainty affects the whole population. We applied an analysis framework that combined morphological diagnostic traits, Bayesian clustering analyses using 14 microsatellite loci, and mtDNA haplotypes to assess the ancestry of all individuals that were alive from 2008 to 2012. Simulated data revealed that our microsatellites could accurately assign an individual a hybrid origin until the second backcross generation, which permitted us to identify a pure group among the older, but still reproductive animals. No wild species has ever survived such a severe bottleneck, followed by hybridization, and studying the recovery capability of the selected pure Alagoas Curassow group might provide valuable insights into biological conservation theory.