Culturing of female bladder bacteria reveals an interconnected urogenital microbiota

• Published in: Nature communications Vol. 9; no. 1; pp. 1557 - 7
• Main Authors: Thomas-White, Krystal, Forster, Samuel C., Kumar, Nitin, Van Kuiken, Michelle, Putonti, Catherine, Stares, Mark D., Hilt, Evann E., Price, Travis K., Wolfe, Alan J., Lawley, Trevor D.
• Format: Journal Article
• Language: English
• Published: London Nature Publishing Group UK 19.04.2018; Nature Publishing Group; Nature Portfolio
• Subjects: 45; 49/22; 49/23; 631/326/2565/2134; 631/326/2565/2142; 631/326/325/2482; 692/4025/1334; Adult; Aged; Aged, 80 and over; Bacteria; Bacteria - classification; Bacteria - genetics; Bacteria - growth & development; Bacteria - isolation & purification; Biodiversity; Bladder; Cohort Studies; Commensals; E coli; Female; Genome, Bacterial; Genomes; Humanities and Social Sciences; Humans; Lactobacillus; Microbiota; Middle Aged; multidisciplinary; Nucleotide sequence; Phylogeny; Science; Science (multidisciplinary); Species diversity; Strains (organisms); Urinary Bladder - microbiology; Urine; Vagina; Vagina - microbiology; Young Adult
• ISSN: 2041-1723; 2041-1723
• DOI: 10.1038/s41467-018-03968-5

Metagenomic analyses have indicated that the female bladder harbors an indigenous microbiota. However, there are few cultured reference strains with sequenced genomes available for functional and experimental analyses. Here we isolate and genome-sequence 149 bacterial strains from catheterized urine of 77 women. This culture collection spans 78 species, representing approximately two thirds of the bacterial diversity within the sampled bladders, including Proteobacteria, Actinobacteria, and Firmicutes. Detailed genomic and functional comparison of the bladder microbiota to the gastrointestinal and vaginal microbiotas demonstrates similar vaginal and bladder microbiota, with functional capacities that are distinct from those observed in the gastrointestinal microbiota. Whole-genome phylogenetic analysis of bacterial strains isolated from the vagina and bladder in the same women identifies highly similar Escherichia coli , Streptococcus anginosus , Lactobacillus iners , and Lactobacillus crispatus , suggesting an interlinked female urogenital microbiota that is not only limited to pathogens but is also characteristic of health-associated commensals. The female bladder seems to harbor a poorly characterized indigenous microbiota. Here, the authors isolate and genome-sequence 149 bacterial strains from catheterized urine of 77 women, generating a culture collection representing two thirds of the bacterial diversity within the samples.