Identification and functional characterization of the AGO1 ortholog in maize

• Published in: Journal of integrative plant biology Vol. 58; no. 8; pp. 749 - 758
• Main Authors: Xu, Dongdong, Yang, Hailong, Zou, Cheng, Li, Wen-Xue, Xu, Yunbi, Xie, Chuanxiao
• Format: Journal Article
• Language: English
• Published: China (Republic : 1949- ) Blackwell Publishing Ltd 01.08.2016; Institute of Crop Science,Chinese Academy of Agricultural Sciences,Beijing 100081,China; John Wiley and Sons Inc
• Subjects: AGO1; Amino Acid Motifs; Arabidopsis - genetics; Base Sequence; Conserved Sequence; functional complementation; Gene Expression Profiling; Gene Expression Regulation, Plant; Genes, Plant; Genetic Complementation Test; Genetic Variation; maize; Mutation - genetics; Phylogeny; Plant Proteins - chemistry; Plant Proteins - genetics; Plant Proteins - metabolism; Plants, Genetically Modified; Sequence Homology, Amino Acid; Stress, Physiological - genetics; Transformation, Genetic; Zea mays - genetics; Zea mays - metabolism; ZmAGO1a; ZmAGO1a; functional complementation; maize; AGO1
• ISSN: 1672-9072; 1744-7909
• DOI: 10.1111/jipb.12467

Eukaryotic Argonaute proteins play primary roles in mi RNA and si RNA pathways that are essential for numerous developmental and biological processes. However, the functional roles of the four Zm AGO1 genes have not yet been characterized in maize（Zea mays L.）. In the present study, Zm AGO1 a was identified from four putative Zm AGO1 genes for further characterization. Complementation of the Arabidopsis ago1-27 mutant with Zm AGO1 a indicated that constitutive overexpression of Zm AGO1 a could restore the smaller rosette, serrated leaves, later flowering and maturation, lower seed set, and darker green leaves at late stages of the mutant to the wild-type phenotype. The expression profiles of Zm AGO1 a under five different abiotic stresses indicated that Zm AGO1 a shares expression patterns similar to those of Argonaute genes in rice, Arabidopsis, and wheat.Further, variation in Zm AGO1 a alleles among diverse maize germplasm that resulted in several amino acid changes revealed genetic diversity at this locus. The present data suggest that Zm AGO1 a might be an important AGO1 ortholog in maize. The results presented provide further insight into the function of ZmAGO1a.