Resistance Evaluation to Sheath Blight in Transgenic Rice Lines

Resistance of forty-one homozygous rice lines transformed with chitinase gene (RC24) and β-1,3 -glucanse gene (β-1,3-Glu) to sheath blight was analyzed by inoculation. Among different lines, the resistance had significant differences according to the result by cluster analysis. The lines could be ca...

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Published inRice science Vol. 11; no. 1; pp. 95 - 100
Main Author LI Ai-hong XU Xin-ping DAI Zheng-yuan CHEN Zong-xiang LI Bao-jian ZHANG Hong-xi PAN Xue-biao
Format Journal Article
LanguageEnglish
Published Lixiahe Agricultural Institute of Jiangsu Province, Yangzhou 225002, China%Lixiahe Agricultural Institute of Jiangsu Province, Yangzhou 225002, China%Bio-Engineering Center,Zhongshan University, Guangzhou 510275, China%Department of Agronomy, Agricultural College, Yangzhou University, Yangzhou 225009, China 2004
Department of Agronomy, Agricultural College, Yangzhou University, Yangzhou 225009, China
Subjects
activity
analysis
blight
chitinase
cluster
lines
resistance
rice
sheath
transgenic
transgenic lines
chitinase activity
rice
cluster analysis
sheath blight resistance
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Summary:Resistance of forty-one homozygous rice lines transformed with chitinase gene (RC24) and β-1,3 -glucanse gene (β-1,3-Glu) to sheath blight was analyzed by inoculation. Among different lines, the resistance had significant differences according to the result by cluster analysis. The lines could be categorized into resistant, moderately resistant, moderately susceptible and susceptible types, while 92.1% of which belonging to moderately resistant or moderately susceptible type. For different resistant or susceptible lines, the resistance to rice sheath blight was remarkable correlated with the chitinase activity of transgenic lines except resistant type lines, in which enzyme activity coded by target gene was lower than moderately resistant type. The chitinase activity of transgenic lines tested at different time after inoculation or different organs of the same plant was uniform, which suggested that the expression of chitinase gene was constitutive in nature. Check varieties' chitinase activity would change at different time after inoculation and reach a peak at sometime, but it had no difference at various parts of the same plant.
Bibliography:33-1317/S
S511
ISSN:1672-6308
1876-4762