Differentiation between serological responses to Brucella suis and Yersinia enterocolitica serotype O[ratio ]9 after natural or experimental infection in pigs

• Published in: Epidemiology and infection Vol. 134; no. 2; pp. 347 - 357
• Main Authors: JUNGERSEN, G., SØRENSEN, V., GIESE, S. B., STACK, J. A., RIBER, U.
• Format: Journal Article
• Language: English
• Published: Cambridge, UK Cambridge University Press 01.04.2006
• Subjects: Animals; Antibodies; Antigens; Bacteriology; Biological and medical sciences; Brucella; Brucella suis; Brucella suis - immunology; Brucella suis - pathogenicity; Brucellosis; Brucellosis - diagnosis; Diagnosis, Differential; Enzyme linked immunosorbent assay; False Positive Reactions; Food; Fundamental and applied biological sciences. Psychology; Herds; Hogs; Infections; Inoculation; Laboratories; Microbiology; Miscellaneous; Serologic Tests; Serology; Swine; Swine Diseases - diagnosis; Yersinia; Yersinia enterocolitica; Yersinia enterocolitica - immunology; Yersinia enterocolitica - pathogenicity; Yersinia Infections - diagnosis; United Kingdom > UK; Denmark; Human; Microbiology; Epidemiology; Yersinia enterocolitica; Vertebrata; Mammalia; Brucellaceae; Swine; Bacteria; Artiodactyla; Serotype; Veterinary; Brucella suis; Ungulata; Brucella melitensis; Humoral immunity; Enterobacteriaceae
• ISSN: 0950-2688; 1469-4409
• DOI: 10.1017/S095026880500511X

False-positive serological reactions (FPSR) due to infections with Yersinia enterocolitica serotype O[ratio ]9 (YeO[ratio ]9) are a problem in tests for brucellosis. In the present study, FPSR in classical and novel tests for brucellosis following experimental infections of pigs with YeO[ratio ]9 were compared with responses of B. suis biovar 2-inoculated pigs. FPSR were limited to 2–9 weeks post-YeO[ratio ]9 inoculation, while B. suis-infected pigs were test-positive throughout the 21-week period of investigation. Although YeO[ratio ]9-inoculated pigs exhibited FPSR in Brucella tests for a limited period of time, the serological responses in a YeO[ratio ]9-purified O-antigen indirect ELISA did not decrease accordingly. Analysis of available cross-sectional serum samples from pig herds naturally infected with YeO[ratio ]9 or B. suis biovar 2 confirmed that the observed difference in the duration of the serological responses between the two infections could be used to discriminate between herds infected with B. suis biovar 2 and YeO[ratio ]9.