Development of embryogenic dogwood cultures and the regeneration of plants

• Published in: Plant Biotechnology Vol. 36; no. 1; pp. 53 - 56
• Main Authors: Gladfelter, Heather J., Wilde, H. Dayton
• Format: Journal Article
• Language: English
• Published: Japan Japanese Society for Plant Cell and Molecular Biology 2019; Japan Science and Technology Agency
• Subjects: Bioengineering; Butyric acid; Cornus florida; Dichlorophenoxyacetic acid; Embryonic growth stage; Embryos; Flowering; Growth regulators; Indole-3-butyric acid; Picloram; Plant growth; plant growth regulator; Plants (botany); Regeneration; Somatic embryogenesis; plant growth regulator; Cornus florida; regeneration; somatic embryogenesis; indole-3-butyric acid
• ISSN: 1342-4580; 1347-6114
• DOI: 10.5511/plantbiotechnology.18.1221a

Somatic embryogenesis in flowering dogwood (Cornus florida) has been achieved, but not the regeneration of plants with active shoot growth. To improve plant regeneration, eight media treatments were tested for induction of somatic embryogenesis from immature zygotic embryos. Somatic embryogenesis was obtained on three media containing the plant growth regulators (PGRs) 0.1 mg l−1 picloram, 2.0 mg l−1 2,4-dichlorophenoxyacetic acid, or 0.1 mg l−1 indole-3-butyric acid (IBA). Somatic embryogenesis was also induced on Woody Plant Medium without PGRs. Overall, 92% of the somatic embryos examined converted into plants with active root and shoot growth. This is the first report of somatic embryo-derived plants of C. florida that have active shoot growth and that could be transferred to soil. Embryogenic suspensions were established from IBA-treated cultures that could serve as a target for C. florida bioengineering.