Enzymatically synthesized glycogen protects inflammation induced by urban particulate matter in normal human epidermal keratinocytes

• Published in: Journal of Clinical Biochemistry and Nutrition Vol. 67; no. 1; pp. 29 - 35
• Main Authors: Kitakaze, Tomoya, Yoshioka, Yasukiyo, Furuyashiki, Takashi, Ashida, Hitoshi
• Format: Journal Article
• Language: English
• Published: Japan SOCIETY FOR FREE RADICAL RESEARCH JAPAN 2020; Japan Science and Technology Agency; the Society for Free Radical Research Japan
• Subjects: Accumulation; Aging; Antioxidants; Aromatic compounds; Cytochrome P450; Cytokines; enzymatically synthesized glycogen; Glycogen; Glycogens; Inflammation; Interleukin 6; Keratinocytes; normal human epidermal keratinocytes; Nuclear transport; Original; Oxidative stress; Particulate emissions; Particulate matter; Phosphorylation; Polycyclic aromatic hydrocarbons; Proteins; Reactive oxygen species; Skin cancer; Starch; Synthesis; Translocation; particulate matter; enzymatically synthesized glycogen; normal human epidermal keratinocytes; inflammation; oxidative stress
• ISSN: 0912-0009; 1880-5086
• DOI: 10.3164/jcbn.20-43

Urban particulate matters (PM) exposure is significantly correlated with extrinsic skin aging signs and skin cancer incidence. PM contains polycyclic aromatic hydrocarbons, and they act as the agonists of aryl hydrocarbon receptor (AhR). Activation of AhR promotes generation of intracellular reactive oxygen species (ROS) and inflammation. Enzymatically synthesized glycogen (ESG), which is synthesized from starch, possesses various functions, such as anti-tumor, anti-obesity and antioxidant. However, the effects of ESG on PM-induced skin inflammation remain unclear. In this study, we investigated whether ESG has a protective effect on PM-induced oxidative stress and inflammation in human epidermal keratinocytes. ESG inhibited PM-induced expression of inflammatory cytokines IL6, TNFA and PTGS2. ESG also inhibited PM-induced phosphorylation of MAPKs and ROS accumulation. However, ESG had no effect on PM-induced expression of CYP1A1, one of the target proteins of AhR. On the other hand, ESG increased nuclear translocation of Nrf2 and expression of antioxidant proteins, HO-1 and NQO1. These results suggest that ESG suppressed PM-induced inflammation by decreasing ROS accumulation through the Nrf2 pathway.