Stage-Specific Expression of Estrogen Receptor Subtypes and Estrogen Responsive Finger Protein in Preimplantational Mouse Embryos

• Published in: ENDOCRINE JOURNAL Vol. 46; no. 1; pp. 153 - 158
• Main Authors: HIROI, HISAHIKO, MOMOEDA, MIKIO, INOUE, SATOSHI, TSUCHIYA, FUJIKO, MATSUMI, HIROTAKA, TSUTSUMI, OSAMU, MURAMATSU, MASAMI, TAKETANI, YUJI
• Format: Journal Article
• Language: English; Japanese
• Published: Japan The Japan Endocrine Society 1999
• Subjects: Animals; Blastocyst - chemistry; DNA-Binding Proteins - genetics; Embryo, Mammalian - metabolism; Embryonic Development; Estrogen receptor subtypes; Estrogen-responsive finger protein; Female; Gene Expression; Male; Mice; Mice, Inbred ICR; Morula - chemistry; Mouse; Oocytes - chemistry; Ovary - chemistry; Polymerase Chain Reaction; Pregnancy; Preimplantation embryos; Receptors, Estrogen - genetics; Ribosomal Proteins - genetics; RNA, Messenger - analysis; Transcription Factors - genetics; Zinc Fingers
• ISSN: 0918-8959; 1348-4540
• DOI: 10.1507/endocrj.46.153

In hope of understanding possible roles of estrogen during early embryogenesis, we examined the expression of both estrogen receptor α (ERα) and ERβ, a recently cloned novel subtype, in mouse oocytes and preimplantation embryos by means of reverse transcription polymerase chain reaction (RT-PCR). To investigate whether estrogen actually exerts its action, we further determined the expression of efp (estrogen-responsive finger protein), a newly characterized estrogen responsive gene belonging to the RING finger family. ERα mRNA was detected in whole ovaries, cumulus-oocyte complexes, denuded oocytes, 2-cell and 4-cell embryos, whereas it was undetected in 8-cell embryos. Interestingly it reappeared in morulae and blastocysts. ERβ mRNA was detected similarly to ERα except for the absence of ERβ mRNA in morulae. The efp mRNA was detected in whole ovaries, cumulus-oocyte complexes, 4-cell embryos, morulae and blastocysts. The stage specific expression of ERα and ERβ along with detection of the product of the estrogen responsive gene in early preimplantation embryos may indicate the possible physiological roles of estrogen in early embryogenesis.