In vitro Antibacterial Effect of Polyglycerol Monolaurates against Gram-Bacteria and Understanding the Underlying Mechanism

Polyglycerol monolaurates are generally recognized as safe food additives and are commonly used as food emulsifiers. In this study, the antimicrobial effect of four polyglycerol monolaurates on two Gram-positive bacteria (Staphylococcus aureus and Bacillus subtilis) and two Gram-negative bacteria (E...

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Bibliographic Details
Published inJournal of Oleo Science Vol. 70; no. 4; pp. 571 - 580
Main Authors Zhao, Feifei, Wang, Wenyue, Zhang, Guiju, Zhang, Jieying, Liu, Changyao, Xu, Baocai
Format Journal Article
LanguageEnglish
Japanese
Published Japan Japan Oil Chemists' Society 01.01.2021
Japan Science and Technology Agency
Subjects
Antiinfectives and antibacterials
Antimicrobial agents
antimicrobial effect
Bacteria
Cell membranes
Coliforms
E coli
Food additives
food-related bacteria
Gram-positive bacteria
Morphology
Permeability
polyglycerol monolaurate
Polyglycerols
Pseudomonas aeruginosa
Triglycerides
underlying mechanism
antimicrobial effect
food-related bacteria
polyglycerol monolaurate
underlying mechanism
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Summary:Polyglycerol monolaurates are generally recognized as safe food additives and are commonly used as food emulsifiers. In this study, the antimicrobial effect of four polyglycerol monolaurates on two Gram-positive bacteria (Staphylococcus aureus and Bacillus subtilis) and two Gram-negative bacteria (Escherichia. coli and Pseudomonas aeruginosa) were investigated. The minimum inhibitory concentration (MIC) of diglycerol monolaurate (PG2ML), triglycerol monolaurate (PG3ML), hexaglycerol monolaurate (PG6ML), and decaglycerol monolaurate (PG10ML) against S. aureus was 0.16, 0.32, 0.63, and 1.25 mg/mL, respectively. The MIC of PG2ML, PG3ML, PG6ML, and PG10ML against B. subtilis was 0.32, 0.63, 1.25, and 3.75 mg/mL, respectively. No apparent antimicrobial effect of these four polyglycerol monolaurates on E. coli and P. aeruginosa was observed even up to 10.00 mg/mL. The underlying mechanism was investigated by assessing cell membrane permeability, the integrity of cell membrane, and morphology. We concluded that polyglycerol monolaurates might eliminate Gram-positive bacteria by disrupting the cell membrane, thereby increasing cell membrane permeability, releasing the cellular contents, and altering the cell morphology.
ISSN:1345-8957
1347-3352
DOI:10.5650/jos.ess20274