基于竞争性杂交方法的猪-肠道微生物特异性互作靶点发掘

动物肠道中存在着一些参与猪肠道微生物互作的基因,这些基因具有一定的宿主特异性,利用其设计分子标记能准确识别粪便污染来源。该文共采集6个物种（猪、牛、羊、鸡、鸭、鹅）的145个粪便样品,提取其DNA后利用竞争性杂交的基因片段富集方法（genome fragment enrichment,GFE）,靶向筛选参与猪肠道微生物互作的特异性基因。经BLASTX分析发现,82%的猪特异性非冗余DNA片段存在相似序列,以拟杆菌纲（Bacteroidetes）（43.2%）、梭菌纲（Clostridia）（19.5%）、芽孢杆菌纲（Bacilli）（8.6%）相似序列为主。从蛋白质功能方面分析,61.5%的非...

Full description

Saved in:
Bibliographic Details
Published in浙江大学学报（农业与生命科学版） Vol. 43; no. 2; pp. 163 - 172
Main Author 樊丽华 莫虹斐 张晓峰 帅江冰 陈青
Format Journal Article
LanguageChinese
Published 浙江工商大学食品与生物工程学院,杭州,310035%浙江省检验检疫科学技术研究院,杭州,310016 2017
Subjects
功能注释
特异性基因组片段富集
猪特异性分子标记
竞争性杂交
非点源污染
功能注释
特异性基因组片段富集
function annotation
非点源污染
swine-specific molecular marker
猪特异性分子标记
竞争性杂交
non-point source pollution
competitive DNA hybridization
genome fragment enrichment
Online AccessGet full text
ISSN1008-9209
DOI10.3785/j.issn.1008-9209.2016.06.151

Cover

More Information
Summary:动物肠道中存在着一些参与猪肠道微生物互作的基因,这些基因具有一定的宿主特异性,利用其设计分子标记能准确识别粪便污染来源。该文共采集6个物种（猪、牛、羊、鸡、鸭、鹅）的145个粪便样品,提取其DNA后利用竞争性杂交的基因片段富集方法（genome fragment enrichment,GFE）,靶向筛选参与猪肠道微生物互作的特异性基因。经BLASTX分析发现,82%的猪特异性非冗余DNA片段存在相似序列,以拟杆菌纲（Bacteroidetes）（43.2%）、梭菌纲（Clostridia）（19.5%）、芽孢杆菌纲（Bacilli）（8.6%）相似序列为主。从蛋白质功能方面分析,61.5%的非冗余序列功能明确,有7.6%的序列与信息贮存及加工有关,12.8%的序列与细胞加工及信息传导有关,22%的序列与代谢有关,其中,碳水化合物和氨基酸的转移代谢相关序列含量最为丰富,均占总特异性序列的6.3%。研究发现,能够编码拟杆菌纲（Bacteroidetes）和梭菌纲（Clostridials）等表面蛋白、膜分泌蛋白及碳水化合物代谢蛋白的相关基因可作为猪特异性分子标记筛选的靶点。
Bibliography:33-1247/S
Summary Due to the rapid development of livestock breeding and poultry raising, non-point source pollution has become a significant threat to environmental management and aquaculture industry development, as well as to human health in the last few decades. Therefore, it is particularly urgent to establish a monitoring method that can be used as the efficient indicator of fecal pollution with high sensitivity and strong specificity. In animal guts, genes which are directly involved in bacterium-host interactions may display increased level of host-associated genetic variation, making them promising candidates for fecal source tracking. Specific markers targeting bacterium-host interaction genes for human, cattle and chicken were reported previously; however, swine-specific marker for fecal source tracking has not been found yet. We applied a genome fragment enrichment （GFE） method to enrich swine-specific metagenomic regions that differ from those of other animal species. Briefly, a portion of swine f
ISSN:1008-9209
DOI:10.3785/j.issn.1008-9209.2016.06.151