Separation of Polyprenyl Phosphate Oligomerhomologues by Reversed-Phase Ion-Pair High-Performance Liquid Chromatography

A simple and rapid method of reversed-phase ion-pair high-performance liquid chromatography (HPLC) with UV-detection on a common C18 column in an isocratic mode with the addition of tetrabutylammonium phosphate for the separation of polyprenyl phosphate oligomerhomologues has been developed. The met...

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Bibliographic Details
Published inAnalytical Sciences Vol. 28; no. 10; pp. 1021 - 1023
Main Authors KOZLOV, Vyacheslav V., DANILOV, Leonid L.
Format Journal Article
LanguageEnglish
Published Singapore The Japan Society for Analytical Chemistry 2012
Springer Nature Singapore
Japan Science and Technology Agency
Subjects
Analytical Chemistry
Chemistry
Chromatography, High Pressure Liquid - methods
Chromatography, Reverse-Phase - methods
Drugs
Leaves
Liquid chromatography
Mathematical analysis
Morus - chemistry
Needles
Organophosphates - isolation & purification
Phosphates
Plant Leaves - chemistry
Polymers - isolation & purification
Separation
Veterinary
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Summary:A simple and rapid method of reversed-phase ion-pair high-performance liquid chromatography (HPLC) with UV-detection on a common C18 column in an isocratic mode with the addition of tetrabutylammonium phosphate for the separation of polyprenyl phosphate oligomerhomologues has been developed. The method was successfully applied to assay the composition of polyprenyl phosphates obtained from polyprenols isolated from mulberry (Morus alba) leaves and fir (Abies sibirica) needles (the active components of veterinary drugs Gamapren and Phosprenyl correspondingly).
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ISSN:0910-6340
1348-2246
DOI:10.2116/analsci.28.1021