Liposomal glyco-microarray for studying glycolipid–protein interactions

• Published in: Analytical and bioanalytical chemistry Vol. 404; no. 1; pp. 51 - 58
• Main Authors: Ma, Yong, Sobkiv, Irena, Gruzdys, Valentinas, Zhang, Hailong, Sun, Xue-Long
• Format: Journal Article
• Language: English
• Published: Berlin/Heidelberg Springer-Verlag 01.07.2012; Springer
• Subjects: Analytical Chemistry; Binding; Biochemistry; Biomedical engineering; Biomolecules; Cellular; Characterization and Evaluation of Materials; Chemistry; Chemistry and Materials Science; Cholera Toxin - chemistry; Cholera Toxin - metabolism; Fluorescence; Food Science; Gangliosides; Glycolipids - chemistry; Glycolipids - metabolism; Immobilization; Kinetics; Laboratory Medicine; Lectins; Liposomes; Liposomes - chemistry; Membrane lipids; Microarray Analysis - instrumentation; Microarray Analysis - methods; Monitoring/Environmental Analysis; Original Paper; Plant Lectins - chemistry; Plant Lectins - metabolism; Plant lipids; Protein Binding; Screening; Strategy; Toxins; Liposome; Lectin; Toxin; Glycoarray; Glycolipid
• ISSN: 1618-2642; 1618-2650
• DOI: 10.1007/s00216-012-6096-2

A microarray enables high-throughput interaction screening of numerous biomolecules; however, fabrication of a microarray composed of cellular membrane components has proven difficult. We report fabrication of a liposomal glyco-microarray by using an azide-reactive liposome that carries synthetic and natural glycolipids via chemically selective and biocompatible liposome immobilization chemistry. Briefly, liposomes carrying anchor lipid dipalmitoylphosphatidylethanolamine (DPPE)–PEG 2000 –triphenylphosphine and ganglioside (GM1 or GM3) were prepared first and were then printed onto an azide-modified glass slide so as to afford a liposomal glyco-microarray via Staudinger ligation. Fluorescent dye release kinetics and fluorescence imaging confirmed successful liposome immobilization and specific protein binding to the intact arrayed glycoliposomes. The liposomal glyco-microarray with different gangliosides showed their specific lectin and toxin binding with different binding affinity. The azide-reactive liposome provides a facile strategy for fabrication of either a natural or a synthetic glycolipid-based membrane-mimetic glycoarray. This liposomal glyco-microarray is simple and broadly applicable and thus will find important biomedical applications, such as studying glycolipid–protein interactions and toxin screening applications. Figure Liposome immobilization format provides a facile strategy for glycolipid-based membrane-mimetic glycoarray fabrication