Application of latex beads agglutination test for the detection of the antibody against virus-infection-associated (VIA) antigen of foot-and-mouth disease (FMD) virus

• Published in: Journal of Veterinary Medical Science Vol. 62; no. 7; pp. 805 - 807
• Main Authors: Sugimura, T. (Kagoshima Univ. (Japan). Faculty of Agriculture), Suzuki, T, Chatchawanchonteera, A, Sinuwonkwat, P, Tsuda, T, Murakami, Y
• Format: Journal Article
• Language: English
• Published: Japan JAPANESE SOCIETY OF VETERINARY SCIENCE 01.07.2000; Japan Science and Technology Agency
• Subjects: AGGLUTINATION TESTS; Animals; ANTIBODIES; Antibodies, Viral - blood; Antibody Formation; ANTIGENS; Antigens, Viral - immunology; APHTHOVIRUS; Aphthovirus - isolation & purification; Cattle; Cattle Diseases - diagnosis; Cattle Diseases - immunology; DOMESTIC ANIMALS; foot-and-mouth disease; Foot-and-Mouth Disease - diagnosis; Foot-and-Mouth Disease - immunology; latex beads agglutination test; Latex Fixation Tests - methods; Latex Fixation Tests - veterinary; Swine; Swine Diseases - diagnosis; Swine Diseases - immunology; Time Factors; VIA antigen; Viral Nonstructural Proteins - immunology
• ISSN: 0916-7250; 1347-7439
• DOI: 10.1292/jvms.62.805

Latex beads agglutination (LA) for the detection of the antibody against virus infection-associated (VIA) antigen of foot-and-mouth disease (FMD) virus was estimated using experimentally infected animals. The VIA antibody titer by the LA test were compared with the neutralization titer and the titer by agarose gel diffusion (AGD) test, which has been used as a standard method for VIA antibody titration. The latex beads were coated with VIA antigen in carbonate buffer solution (0.5M, pH9.6) for the test. The sensitivity of the LA test was clearly higher than that of the AGD test in the results for cattle and swine infected experimentally. The antibody was detected in the bovine serum obtained at the 13th week after inoculation by the LA but not by the AGD test. The LA test appears to be simple, rapid and sensitive for the detection of the antibody of FMD virus in the surveillance of FMD and the FMD quarantine of imported animals.